摘要
目的观察ω-3多不饱和脂肪酸（PUFA）对严重烫伤大鼠肺部炎症的影响并探讨其机制。方法72只成年SD大鼠按照随机数字表法分成3组：似伤组（A组，8只）、ω3PUFA治疗组（B组，32只）、中长链脂肪酸治疗组（C组，32只）。A组大鼠浸入32℃水中模拟烫伤，伤后不予其他处理；B、c组制成30％TBSAm度烫伤，伤后5min内，经尾静脉分别注射100g／L ω-3PUFA（1mL／kg）和200g／L中长链脂肪酸注射液（2mL／kg），两者热量等值，之后每天注射1次，连续10d．检测A组大鼠伤后即刻及B、C组伤后1、4、7、10d血清TNF—a、肺组织匀浆巨噬细胞炎症蛋门1d（MIP—1a）水平，免疫组织化学染色法观察肺组织NF—KBp65及巨噬细胞游走抑制因子（MIF）表达情况。对实验数据进行t检验。结果伤后所有时相点3组大鼠各项检测指标比较，均为B、c组叫显高于A绀，t值为3．411～8．782，P值均小于0．01。伤后4、7、10d，B组各项指标水平明显低于c组，t值为2．321～2．785，P值均小于0．05。A组TNF—a为（0．96±0．32）ng／mL、MIP—1a为（76±16）pg／mL、NF—CBp65为（0．24±0．03）分、MIF为（1．31±0．03）分；B组以上指标伤后7d达峰值，分别为（2．43±0．32）ng／mL、（210±56）pg／mL、（4．23±2．15）分、（4．69±1．83）分；C组以上指标伤后7d为（3．15±0．54）ng／mL、（274±64）pg／mL、（5．15±2．31）分、（5．37±2．16）分。结论ω-3PUFA能降低烫伤大鼠血清TNF—a含最和肺组织MIP-1a、NF—KBp65、MIF水平，对肺组织有一定保护作用。
Objective To study the effect of ω-3 polyunsaturated fatty acids （ ω-3 PUFA） on in- flammation in lung tissue of rats with severe scald and its mechanism. Methods Seventy-two aduh SI） rats were divided into sham scald group （SS, n = 8） , treatment group 1 （TI , n = 32） , treatment group 2 （T2, n = 32） according to the random numher table. Rats in T1 group and T2 group were inflicted with 30% TBSA full-thickness scald, and then they were respectively injected with 100 g/L ω-3 PUFA （1 mL/kg） and 200 g/L long-chain fatty acid （2 mL/kg） via tail vein within 5 minutes after hurn. The above two fatty acids with equivalent calories were continuously injected for 10 days （once a day）. On post burn day （PBD） 1, 4, 7, and 10, serum level of TNF-ω and level of macrophage inflammatory protein la （MIP-1a） in lung homogenate of TI and T2 groups were detected, the levels of NF-KBp65 and macrophage migration inhibitory factor （MIF） in lung tissue of TI and T2 groups were observed with immunohisluehemieal staining （recorded as score）. Ahove-meutiuned parameters were also determined in SS group. Data were processed with t test. Results The levels of 4 parameters in TI and T2 groups on PBD I , 4, 7, 10 were higher than those in SS group （with t values from 3. 411 to 8. 782, P values all below 0.01 ） , and those in TI group on PBD 4, 7, 10 were lower than those in T2 group （with t values from 2. 321 to 2. 785, P values all below 0.05）. The serum level of TNF-a and levels of MIP-1a, NF-KBp65, and MIF in lung tissue in SS group was respectively （0.96 ±0.32） ng/ml,, （76 ± 16） pg/mL, 0.24 ±0.03, 1.31 ± 0.03, and those in TI and T2 groups all peaked on PB1） 7 [（2.43±0.32） ng/mL, （210 ±56） pg/mL, 4.23 ±2. 15, 4.69± 1.83; （3.15±0.54） ng/mL, （274＋64） pg/mL, 5.15±2.31, 5.37±2.16]. Conclusions co-3 PUFA can effectively reduce serum level of TNF-a and levels of MIP-1a , NF-KBp65, and MIF in lung lissue of rats with severe scaht, showing that it has a protective effect against injury of lung tissue.
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2011年第5期358-362,共5页
Chinese Journal of Burns
基金
基金项目：安徽省自然科学基金（090413267X）
关键词
烧伤
脂肪酸类
不饱和
肿瘤坏死因子A
巨噬细胞游走抑制因子
肺
炎
症
NF-KB
巨噬细胞炎性蛋白质1
Burns
Fatty acids, unsaturated
Tumor necrosis factor-alpha
Macrophage migration inhibitory factors
Lung
Inflammation
Nuclear factor kappa B
Macrophage inflammatoryprotein 1
