摘要
目的观察信号调节蛋白（SIRP）a1在自身免疫性肝炎（AIH）中的表达情况，并探讨其与肝炎活动程度之间的关系。方法用免疫组织化学检测的方法对33例AIH患者肝脏穿刺获得的标本和10例正常肝组织标本中SIRPa1的表达情况进行检测，用秩和检验作统计学分析。结果SIRPd1在AIH标本中呈弱阳性或阳性表达，阳性着色定位于肝脏血窦内枯否细胞的细胞质，呈局灶性分布。SIRPa1在正常肝组织中无表达；在轻度A1H中呈阴性或弱阳性表达，阳性率为36．4％（4／11）；在中度AIH中呈阳性或强阳性表达，阳性率为84．2％（16／19）。轻、中、重度AIH总体比较，X^216．129，P〈0．01，差异有统计学意义。轻、中度AIH之间比较，W=82．5，P〈0．01，差异有统计学意义。而轻、重度AIH之间及中、重度AIH之间比较，差异均无统计学意义（P值均〉0．05）。结论SIRPa1作为一种负向调控因子在AIH的枯否细胞中表达，并且与AIH的严重程度有一定相关陛。
Objective To observe the expression change of signal regulatory proteina1 （SIRPctl） in autoimmune hepatitis （AIH） and approach the relationship between SIRPctl and the extent of inflammation. Methods Immunohistochemistry is used to detect the expression of SIRPa1 in the paraffin section preparations of 33 AIH and 10 normal hepatic tissue. Results SIRPa1 is positive or weakly positive expressed in AIH. The staining is localized in the cytoplasm of Kupffer cells in the hepatic sinusoid with focal distribution. It is negative in normal hepatic tissue. In light AIH, it is negative or weakly positive expressed with a 36.4 percent of the positive rate （4/11）. The positive or strong positive expression is found in the moderate AIH with an 84.2 percent of the positive rate（16/19）. There is statistical significance between both light AIH, moderate AIH and severe AIH （ P〈 0.001） and moderate AIH and light AIH （P〈 0.001）. There is no statistical significance between both light AIH and severe AIH （P = 0.145 ） and moderate AIH and severe AIH （P = 0.084）. Conclusions As a negative regulatory factor, the expression of SIRPa1 in hepatic sinusoid Kupffer cells is some associated with the extent of AIH.
出处
《中华肝脏病杂志》
CAS
CSCD
北大核心
2011年第10期779-781,共3页
Chinese Journal of Hepatology
关键词
肝炎
自身免疫性
枯否细胞
信号调节蛋白
Hepatitis, autoimmune
Kupffer cells
Signal regulatory proteins.
