摘要
目的:建立HPLC法测定制痂酊中儿茶素和表儿茶素含量的方法,同时考察不同剂量^(60)Co辐照对其含量影响。方法:色谱柱:Kromasil C_(18)(250 mm×4.6 mm,5μm),流动相:2.2%冰醋酸-乙腈(86:14),流速:0.8 ml·min^(-1),检测波长:280nm,柱温:35℃。结果:儿茶素和表儿茶素分别在1.025～12.813μg,0.196～2.450μg范围内线性良好,加样回收率分别为98.0%,99.8%(n=5);^(60)Co辐照剂量为3 kGy时,儿茶素和表儿茶素含量分别减少2.77%,3.43%,随着辐照剂量的增加,两者含量有逐渐下降的趋势。结论:对单批次样本测定表明:^(60)Co辐照对制痂酊中儿茶素和表儿茶素的含量有一定影响,但在低剂量(≤3 kGy)时影响不显著。
Objective ： To establish an HPLC method for the simultaneous determination of eateehin and epieateehin in Zhijia tincture and study the effects of different dose 6o Co irradiation on the contents. Method： Kromasil C18 column （250 mm× 4. 6 mm,5 μm） was used. The mobile phase consisted of 2. 2% aeetum-aeetonitrile （86： 14） with the flow rate of 0. 8 ml·min^-1. The detection wavelength was set at 280 nm and column temperature was 35 ℃. Result： Good linearity was obtained over the range of 1. 025-12. 813 μg for catechin and 0. 196-2. 450 μg for epicatechin with the average recovery of 98.0% and 99. 8% , respectively （n = 5 ）. The content of eateehin and epieateehin in Zhijia tincture （irradiated by 3 kGy of ^60Co） was decreased 2. 77% and 3.43% ,respectively,and the contents had declining trend with the increase of irradiation dose. Conclusion：^ 60Co irradiation has influence on the contents of eatechin and epieateehin in Zhijia tincture, however the influence is not significant when the irradiation dose is less than 3 kGy.
出处
《中国药师》
CAS
2011年第10期1463-1464,共2页
China Pharmacist
