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蛋白激酶C对转录延伸正调节因子b(P-TEFb)的影响 被引量：1

The regulation of P-TEFb by PKC
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摘要 本文探讨了PKC的4种亚型α、βⅡ、γ和λ对P-TEFb各蛋白组分的表达影响.成功构建了带有HA标签的pcDNA3.1-PKC的各亚型真核表达载体,并转染HEK293细胞株,用Western bolt检测其表达情况,发现这四种PKC亚型在细胞中表达良好。将PKCβⅡ或者PKCλ转染进入HEK293细胞株,发现其可使CDK9和Cyclin T蛋白表达量升高;但在细胞中高表达PKCα和PKCγ,对P-TEFb各组分表达量没有影响.表明PKC可通过提高P-TEFb的组分表达量,从而上调某些基因的表达. The impact of PKC on the expression of P-TEFb was explored in this paper. PKCα,βII. γandλ were ligated into the eukaryotic expression vector pcDNA3. 1, and were transfected into the HEK293 cell line. The identity of PKCs was confirmed by anti-HA Western blot analysis. The PKC proteins were well expressed. When PKCfllI or PKC2was over-expressed, the expression level of CDK9 and Cylin T increased. PKCα and PKCγ had no impact on the level of P-TEFb. The conclusion was that PKC can increase the expression level of certain genes by increasing the amount of P-TEFb.
作者 任浙丹 黄迎彬 杨洁 赵长宏 田平平 吴传芳
机构地区 四川大学生命科学院功能基因组实验室
出处 《四川大学学报（自然科学版）》 CAS CSCD 北大核心 2011年第5期1204-1208,共5页 Journal of Sichuan University(Natural Science Edition)
基金 国家自然科学基金(30970625)
关键词 蛋白激酶C(PKC) 转录调控 Western免疫印迹 转录延伸正调节因子b(P—TEFb) protein kinase C, transcription regulation, western blot, positive transcription elongation factor b（P-TEFb）
分类号 Q71 [生物学—分子生物学]
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参考文献12

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四川大学学报（自然科学版）
2011年 第5期

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