摘要
通过探讨顺-3-己烯醇、芳樟醇氧化物、芳樟醇、水杨酸甲酯、香叶醇、苯甲醇和苯乙醇7种茶叶游离态香气组分和糖苷类香气前体对茶炭疽病菌、茶云纹叶枯病菌、茶轮斑病菌和茶赤叶斑病菌4种致病菌的抑制作用,以及真菌侵染引发的茶树(Camellia sinensis)内源β-樱草糖苷酶、β-木糖苷酶、β-葡萄糖苷酶I、β-葡萄糖苷酶II、β-葡萄糖基转移酶基因的表达差异,结果表明:7种游离态香气组分和糖苷类香气前体对4种致病菌均有明显的抑制作用,其中香叶醇的抑制作用最强,在浓度为0.1mg.mL-1时即对茶炭疽病菌的抑制率达到100%。实时定量PCR结果显示:真菌侵染可不同程度诱导茶树内源糖苷酶和β-葡萄糖基转移酶基因的表达上调,且上调多发生于染病初期。
We tested the antifungal activities of 7 free-form tea aroma components（cis-3-hexenol,linalool oxides,linalool,methyl salicylate,geraniol,benzyl alcohol and phenylethanol） and glycosidical aroma precursors against Gloeosporium theae-sinensis Miyak,Colletotrichum camelliae Masse,Pestalotiopsis theae Sawada and Phyllosticta theicola Petch.We also investigated the differential gene expression of tea endogenous β-primeverosidase,β-xylosidase,β-glucosidase I,β-glucosidase II and β-glucosyltransferase induced by pathogenic fungi infection.The 7 free-form aroma components and glycosidical aroma precursors conferred antifungal activities against 4 pathogenic fungi.Geraniol showed the strongest activity,and its inhibition rate to G.theae-sinensis Miyak was 100% at 0.1 mg.mL-1.Real-time quantitative PCR revealed that pathogenic fungi infection promoted the expression of endogenous glycosidases and β-glucosyltransferase differently and that promotion usually occurred with slight infection.
出处
《植物学报》
CAS
CSCD
北大核心
2011年第5期552-559,共8页
Chinese Bulletin of Botany
基金
国家自然科学基金(No.30872057)
关键词
抑菌
香气
茶
差异表达
糖苷酶
antifungal
aroma
Camellia sinensis
differential gene expression
glycosidases
