摘要
背景:骨髓间充质干细胞是多能干细胞,同时可参与免疫调节反应,而成人及引产胎儿骨髓均是间充质干细胞的重要来源。目的:通过引产儿与正常成人骨髓间充质干细胞的细胞形态、免疫表型、增殖活性及分泌细胞因子的比较,揭示二者生物学特点的差异。方法:采用密度梯度离心法分离引产儿与正常成人骨髓单个核细胞,通过贴壁法培养骨髓间充质干细胞,收集传3代后的骨髓间充质干细胞,流式细胞术鉴定其免疫表型、MTT比色法检测其增殖活性以及用ELISA法检测细胞培养上清液中白细胞介素6、血小板源性生长因子和表皮生长因子的水平。结果与结论:引产儿组和正常成人组骨髓间充质干细胞形态相似、表型相同,分泌细胞因子水平无显著差异,但引产儿细胞增殖活性显著高于正常成人。提示引产儿与正常成人骨髓间充质干细胞除增殖活性有差异外,其余生物学特点相似,引产儿可以为间充质干细胞研究提供新的骨髓来源。
BACKGROUND： Bone marrow-derived mesenchymal stem cells are pluripotent cells and can be involved in immune response. Fetal and adult bone marrow is both important sources for mesenchymal stem cells. OBJECTIVE： To reveal the differences of bone marrow derived mesenchymal stem cells from aborted fetus and normal adult subjects by comparing the biological characters （including morphology, immunophenotype, proliferative activity and cytokines secretion）. METHODS： Bone marrow mononuclear cells of aborted fetus and normal adult subjects were separated by density gradient centrifugation. Bone marrow derived mesenchymal stem cells were cultured with the adherent method. Cells at passage 3 and their culture supernatants were collected. Flow cytometry analysis was used to examine the immunophenotype. The proliferation activity of cells was measured by MTT colorimetric assay. ELISA was used to measure the concentrations of cytokines secreted from bone marrow derived mesenchymal stem cells in the two groups. RESULTS AND CONCLUSION： Cells from both groups have the same morphology, immunophenotype and the levels of cytokines secretion. But in comparison with normal adult subjects, bone marrow derived mesenchymal stem cells from aborted fetus showed higher proliferative activity. Bone marrow derived mesenchymal stem cells from aborted fetus and normal adult subjects have the similar biological characteristics except the proliferative activity. Aborted fetus can provide bone marrow for research of stem cells.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第32期5928-5931,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金资助项目(30972657)
课题名称:银屑病患者骨髓造血干细胞定向分化为T细胞过程Notch信号转导通路的动态研究~~
