摘要
目的探讨高氟水型地区男性不育患者血浆髓过氧化物酶(MPO)、谷胱甘肽硫转移酶(GSTs)活力变化及内分泌功能。方法对62例男性不育患者以及70例正常生育组采用CASAS-QH-Ⅲ计算机辅助精液分析仪进行精液质量分析;采用比色法分别测定血浆髓过氧化物酶、谷胱甘肽硫转移酶活力;采用放射免疫法测定血清黄体生成素(LH)、卵泡刺激素(FSH)和睾酮(T)含量。结果高氟水型地区男性不育患者精子密度、精子活力与生育组比较差异有统计学意义(P<0.05);血浆MPO活力与生育组比较差异有统计学意义(P<0.05);血浆GSTs活力与生育组比较差异有统计学意义(P<0.01);血清黄体生成素(LH)、卵泡刺激素(FSH)较生育组显著升高(P<0.01),血清辜酮(T)水平显著降低(P<0.01)。结论过量氟可能使体内自由基代谢失衡,从而影响成年男性的生殖及内分泌功能。
Objective To investigate changes in myeloperoxidase（MPO）,glutathione S-transferases（GSTs）,and endocrine function in the plasma of male adults who live in areas with highly fluoridated water.Methods CASAS-QH-Ⅲ was used to analyze semen quality in 62 sterile males and 70 normal sterile males.Plasma MPO and GST activity and endocrine function were determined by colorimetric analysis with a spectrophotometer.The serum levels of LH,FSH,and T were measured by radioimmunoassay.Results The sperm density and motility differed significantly（P0.05） between sterile males and normal males.Sterile males had greater plasma MPO activity than did normal males,and the difference was statistically significant（P0.05）.Sterile males had less GST activity than did normal males（P0.05）.The levels of LH and FSH were markedly higher（P0.01） than those in normal males,and the level of T was significantly lower（P0.01）.Conclusion Excessive fluorine intake may cause unbalanced free radical metabolism.This indicates that high fluorine levels may affect reproduction and endocrine function in the male human body.
出处
《中国病原生物学杂志》
CSCD
2011年第9期661-662,678,共3页
Journal of Pathogen Biology
基金
山东省自然科学基金项目(No.ZR2010HL020)
关键词
高氟
髓过氧化物酶
谷胱甘肽硫转移酶
男性不育
High levels of fluorine
myeloperoxidase
glutathione S-transferase
male sterility
