摘要
目的筛选肠型胃癌组织异常表达的miRNAs。方法采用miRCURYTM基因芯片(v.14.0)分析6例肠型胃癌组织和邻近非肿瘤组织之间差异表达的miRNA,设定平均上升或下降倍数大于2倍和P值小于0.01为显著性差异标准;选取部分基因芯片分析中呈异常表达的miRNAs,采用实时荧光定量PCR(RT-qPCR)方法在29例肠型胃癌组织和邻近非肿瘤组织中进一步检测,并对两者结果进行相关性分析。结果基因芯片结果显示,40个miRNAs呈显著表达上调,其中24个基因已证实在胃癌癌组织中表达升高;36个miRNAs呈显著表达下调,其中19个已报道在胃癌组织中异常表达降低。用RT-qPCR检测6个在基因芯片分析中表达上调的miRNAs和5个在基因芯片分析中表达下调的miRNAs,结果与基因芯片分析结果相一致,两种方法分析的结果呈高度正相关(P<0.01)。结论该研究鉴定了肠型胃癌组织中一系列新的异常表达的miRNAs,为进一步研究提供了基础。
Objective To screen out the aberrantly expressed miRNAs in the intestinal type gastric cancer.Methods The miRNA expression levels of 6 cases of intestinal type gastric cancer tissues and their adjacent non-tumour tissues were evaluated with miRCURYTM LNA microRNA array（v.14.0）,and the average level increased（or decreased） more than 2 folds and P value less than 0.01 were set as cutoff values.Part of the aberrantly expressed miRNAs revealed by microRNA array were selected and further analyzed in 29 cases of intestinal type gastric cancer tissues and their adjacent non-tumour tissues using quantitative real-time PCR（RT-qPCR）,and the correlation between the two results was analyzed.Results As revealed by miRNA microarray analysis,40 mRNA presented significant up-regulated expression,of which 24 genes had been found to be increased in gastric cancer tissue previuosly;36 mRNA showed significant down-regulated expression,of which 19 mRNA had been reported to be abnormally decreased in gastric cancer previously.Six up-regulate miRNAs and 5 down-regulated miRNAs in the microRNA array analysis were examined by RT-qPCR,and the results were consistent with the results of the microRNA array analysis.There was a significant positive correlation between the two methods（P0.01）.Conclusions Our study identifies a series of new miRNA aberrantly expressed in intestinal type gastric cancer and thereby provides a basis for further study.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2011年第9期951-955,共5页
China Journal of General Surgery
基金
国家自然科学基金资助项目(30271516
30800518/H1617
81072038/H1617)
