摘要
建立固相萃取-气相色谱检测水体中6种邻苯二甲酸酯类物质的方法。采用正交设计确定最佳固相萃取条件为洗脱剂正己烷-丙酮(30:1,V/V)、洗脱体积6mL、洗脱速率2mL/min、上样速率8mL/min,采用氢火焰离子化检测器的气相毛细管色谱。结果表明:邻苯二甲酸酯的检测限为1.4～3.6μg/L,线性范围在1～80mg/L之间,相关系数为0.995以上;低、高质量浓度加标回收率在72%～100%之间,相对标准偏差均小于10%。采用此方法对广东省12个水产养殖基地的养殖水进行邻苯二甲酸酯类物质测定,发现所有检测水样中均有邻苯二甲酸酯类物质残留(4.2～171.5μg/L),其污染程度与地域密切相关。
A method for the simultaneous determination of 6 phthalic scid esters（PAEs） aquaculture in water by solid phase extraction（SPE） and gas chromatography detection was developed.The SPE conditions were optimized using orthogonal array design as follows： n-hexane-acetone（30：1,V/V） as elution solvent at a flow rate of 2.0 mL/min,elution volume 6 mL,and sample loading rate 6.0 mL/min.Enriched samples were determined using a capillary gas chromatograph with flame ionization detector.The ldetection limits of 6 PAEs were in the range of 1.4-3.6μg/L.The method displayed a good linearity over the range of 1 to 80 mg/L,with a correlation coefficient of larger than 0.995.The recoveries of the 6 PAEs across low,medium high spike levels were 72%-117% and the relative standard deviations all lower than 10%.The PAEs in 12 samples from typical aquatic breeding areas in Guangdong province were detected by the established method.All samples were found to be polluted by total PAEs concentration in the range of 4.2-171.5 μg/L.Moreover,the contaminations of PAEs were closely associated with geographic areas.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第18期164-167,共4页
Food Science
基金
国家自然科学基金面上基金项目(B5100800)
广东检验检疫局科技计划项目(2006GDK13)
广东省大学生创新实验项目(S1010561061)
关键词
邻苯二甲酸酯
养殖水体
固相萃取
phthalic acid esters
aquaculture water
solid phase extraction
