摘要
以草鱼肾细胞系(CIK)和草鱼呼肠孤病毒为材料,采用噻唑蓝比色法分先给药后感染、先感染后给药、药物与病毒共孵育后感染3组进行抗草鱼呼肠孤病毒药物的筛选,建立了体外抗草鱼呼肠孤病毒药物筛选的细胞模型。先给药后感染试验组的结果表明,穿琥宁在阻断草鱼呼肠孤病毒吸附和侵入细胞时效果最强,其次是利巴韦林和板蓝根,黄芩的阻断效果最弱;而在先感染后给药试验组中,穿琥宁、利巴韦林和黄芩抑制草鱼呼肠孤病毒增殖的作用显著,板蓝根抑制病毒增殖的作用不明显;在药物与病毒共孵育后感染试验组中,利巴韦林对草鱼呼肠孤病毒有一定的直接杀灭作用,黄芩、板蓝根和穿琥宁等的体外杀病毒效果不明显。该模型的建立确认了药物在CIK细胞上具有抑制草鱼呼肠孤病毒的作用,不同给药方式药物的作用效果不同,也为抗病毒药物的筛选提供了有效的技术平台。
By using the staining assay with MTT,drugs against grass carp reovirus（GCRV） were screened on grass carp kidney（CIK） cell line,therefore,a cell model for screening anti-GCRV drugs was established in the CIK cell culture system in vitro.There were three groups,including drugs administrated to the cells before GCRV challenge（GroupⅠ）,drugs administrated to the cells after GCRV infection（GroupⅡ） and drugs directly killing GCRV（GroupⅢ）.Results of GroupⅠ showed that Potassium Dehydroandrograpolide Succinate had the strongest effect in blocking GCRV to attach to and penetrate into the CIK cells,followed by ribavirin and Radix Isatidis,Radix Scutellariae had the least effect among them.Results of Group Ⅱ indicated that ribavirin,Potassium Dehydroandrograpolide Succinate and Radix Scutellariae had significant effects on inhibiting the replication of GCRV,while Radix Isatidis had no apparent effect.Results of GroupⅢ demonstrated that ribavirin had a significant effect in killing GCRV in vitro,while the other three drugs had no impact in killing GCRV in vitro.The model indicated that drugs might inhibit GCRV multiplication and the different effections of the drugs were calculated of CIK cells with different methods.The model also provided an effective platform for the screening of antiviral drugs.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2011年第9期972-978,共7页
Chinese Veterinary Science
基金
国家大宗淡水鱼产业技术体系项目(nycyti-49-16)
农业部公益性行业科研专项(200803013)
