摘要
为克隆出猪巨细胞病毒SC株DNA聚合酶(DPOL)基因并对其进行分析,根据GenBank中的DPOL基因序列(AF268040)设计了1对引物,用PCR法扩增出猪巨细胞病毒SC株DPOL基因,将其克隆到T载体,测序并进行生物信息学分析。测序结果表明,猪巨细胞病毒DPOL基因全长3 024bp,为一个完整ORF,共编码1 008个氨基酸;与参考毒株的核苷酸同源性为98%,系统进化树表明猪巨细胞病毒与人疱疹病毒6型和7型关系最近;运用生物信息学软件推导出该蛋白的基本性质,并预测出高级结构。成功地进行了猪巨细胞病毒SC株DPOL基因的克隆和生物信息学分析,为今后此基因生物学功能和猪巨细胞病毒复制机理的研究以及病毒系统分类工作提供了参考。
This experiment was conducted to obtain and analyze the DNA polymerase（DPOL） gene of porcine cytomegalovirus（PCMV） SC strain.According to the published sequence of DPOL gene of PCMV（accession number AF268040）,a pair of specific PCR primers was designed to amplify the gene sequence,and the target gene was linked into T vector to sequence and analyze this gene.The sequencing analysis revealed that the whole PCMV DPOL gene was an open reading frame（ORF） consisting of 3 024 nucleotides and encoding 1 008 amino acid residues.The nucleotide sequence of PCMV DPOL gene shared approximately 98% identity with the referred sequence.Phylogenetic analysis showed that PCMV had a closely relationship with human herpesvirus 6（HHV6） and HHV7.Lots of physical and chemical properties of the deduced protein from the DPOL gene were performed by several bioinformatics analysis software,and its advanced structure was predicted.The DPOL gene of PCMV was cloned successfully and analyzed by bioinformatics software,which will help to investigate its biological function and replication mechanism and to classify viral system.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2011年第9期886-891,共6页
Chinese Veterinary Science
基金
国家"十一五"科技支撑计划项目(2006BAD06A18)
教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848)
