摘要
目的体内实验研究皮肤源祖细胞对皮肤创面神经修复的影响。方法分离、培养昆明鼠皮肤源祖细胞,并用CM-DiI进行标记。将30只昆明鼠随机分为细胞移植组,单纯培养基组和空白组,通过尾静脉注射观察细胞在体内的迁移,检测创面的愈合率和PGP9.5的表达。结果皮肤源祖细胞表达Fibronectin和Nestin;伤后第1、2周,皮肤源祖细胞逐渐迁移至皮肤全层,并在皮肤毛囊处亦可见;细胞移植组的创面愈合率分别为31.08%±1.74%,64.20%±1.45%明显高于单纯培养基组和空白组(23.85%±1.59%,49.81%±2.10%;22.63%±1.02%,49.51%±1.10%);细胞移植组PGP9.5荧光密度(11.60±1.67,16.60±1.14)明显高于单纯培养基组和空白组(P<0.05)。结论皮肤源祖细胞参与了创面愈合,同时促进了创面处的神经再生,有利于创面神经修复,可能成为修复神经损伤的种子细胞。
Objective To study the effect of Skin-derived precursors （SKPs） on neural repair in skin wounds in vivo. Methods SKPs were isolated, cultured and identified in vitro and were then labeled with CM-DiI. Thirty Kunming mice were divided into 3 groups randomly： cell transplantation, culture media and control groups. The labeled cells were injected to mice in cell transplantation group through vena caudalis. Cell migration, wound healing rate, and the expres- sion of PGP9.5 were detected at 1, 2, 4 weeks post skin wound. Results Fibronectin and Nestin expression was detected in SKP2. At 1, 2 post wound weeks, SKPs gradually migrated to whole layer of skin including the papillae of hair and whisker follicles. The wound healing rates in cell transplantation group were 31.08% ± 1.74% and 64.20% ± 1.45%, which were higher than those in other groups （23.85% ±1.59%,49.81%± 2.10%;22.63% ± 1.02%,49.51% ± 1.10%）, and the fluorescence densities of PGP9.5 were also higher in cell transplantation group than in other groups （P 〈 0.05）. Conclusions SKPs are involved in skin wound healing, and promote nerve regeneration, which may become the seed cells for repairing nerve damage.
出处
《中国神经精神疾病杂志》
CAS
CSCD
北大核心
2011年第9期531-534,共4页
Chinese Journal of Nervous and Mental Diseases
基金
广东省科技计划项目(编号:2010B031100008)
创伤、烧伤与复合伤国家实验室开放基金(编号:SKLKF200808)
广东省自然科学基金博士科研启动项目(编号:8451008901000894)
关键词
神经再生
创面
干细胞
Neural regeneration Wound Stem cell
