摘要
目的观察经皮电刺激对脊髓损伤大鼠脊髓灰质前角及损伤区神经营养因子-3（NT-3）和肿瘤坏死因子-α（TNF-α）表达的影响，并探讨经皮电刺激在脊髓损伤大鼠神经元重建及功能恢复中的作用机制。方法共选取Wistar大鼠48只，采用随机数字表法将其分为模型组及电刺激组。采用Allen’s法将2组大鼠制成T节段不完全脊髓损伤模型，电刺激组于术后给予经皮电刺激治疗。术后每天采用BBB评分对大鼠运动功能进行评定；2组大鼠分别于术后1d、3d、5d及7d时取材，采用免疫组化法观察2组大鼠在不同时间点脊髓中NT-3及TNF-α变化情况。结果2组大鼠术后BBB评分均呈现增加趋势，并且以电刺激组的改善幅度较显著，该组大鼠BBB评分在术后5d及7d时均明显优于模型组（P〈0．05）；免疫组化检测结果显示，术后2组大鼠NT-3免疫阳性表达均持续增加，于术后5d时达到峰值，于术后7d时开始下降，并且电刺激组NT-3阳性表达量在术后5d及7d时均显著高于模型组（P〈0．05）；术后2组大鼠TNF-α免疫阳性表达均随时间进展而逐渐增多，但以电刺激组的增加幅度相对较缓，该组TNF-α免疫阳性表达在术后5d及7d时均显著低于模型组（P〈0．05）。结论经皮电刺激能促进脊髓损伤大鼠NT-3表达，抑制TNF-α表达增加，有助于脊髓损伤大鼠神经元修复、重建及相关功能恢复。
Objective To research the effects of transcutaneous electrical stimulation （TES） on the expres- sion of neurotrophin-3 （NT-3） and tumor necrosis factor-α （TNF-α） in the ventral horn of rats＇ spinal cords and in the injured region after spinal cord injury （SCI） , and to explore the effects of TES on neuron reconstruction and functional recovery and their mechanisms. Methods Forty-eight Wistar rats were selected and divided using stochastic methods into a model group and a TES group. Using Allen＇s method, a complete SCI model was created at T9. Rats of the TES group were given TES treatment. Basso-Beattie-Brasnahan （BBB） ratings were used to evaluate locomotor function. Both groups were sampled at 1, 3, 5 and 7 days after the operation. Immunohistochemical techniques were used to detect the expression of NT-3 and TNF-α in the rats＇ spinal cords at the different time points. Results The post-operative BBB ratings of both groups showed an increasing trend. In the TES group the improvement was significantly better at 5 and 7 days than in the model group. The expression of NT-3 immuno-positive ceils increased in both groups, peaking at 5 days post-operation, then declining at day 7. The expression of NT-3 positive ceils at days 5 and 7 had increased significantly more in the TES group than in the model group. TNF-α immuno-positive expression in- creased with time in both groups, but in the TES group the expression increased substantially less than in the model group. At days 5 and 7 post-operation, the expression was significantly lower than in the model group. Conclusions TES can promote NT-3 expression in rats with SCI, inhibit the increase in TNF--α expression, and aid repair and re- construction of neurons and related functional recovery.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2011年第9期643-647,共5页
Chinese Journal of Physical Medicine and Rehabilitation
基金
四川省科技厅课题（06J13-023）,成都医学院创新实验项目（CX201116）
关键词
脊髓损伤
经皮电刺激
神经营养因子-3
肿瘤坏死因子-Α
Spinal cord injury
Transcutaneous electrical stimulation
Neurotrophin-3
Tumor necrosis factor-α
