摘要
目的:检测CD82、hTERT(人端粒酶逆转录酶)蛋白在阴茎癌组织中的表达情况以及阴茎癌组织中的HPV感染情况,以探讨其与阴茎癌病理分期和分级之间的相互关系以及在腹股沟淋巴结转移中的预测作用。方法:44例阴茎癌患者均接受阴茎部分/全切除和区域淋巴结切除或清扫术。应用免疫组化技术检测阴茎癌中CD82、hTERT蛋白的表达。同时应用PCR技术检测HPV感染情况。结果:CD82、hTERT和HPV DNA在阴茎癌中的阳性率分别为47.7%、38.6%和25.9%。扩增出的HPV DNA均为HPV16型。病理分级和hTERT蛋白的表达与阴茎癌腹股沟淋巴结转移呈正相关(P=0.032,P=0.041);CD82表达与病理分期亦呈正相关(P=0.045);病理分期、CD82表达和HPV-16 DNA阳性率与阴茎癌腹股沟淋巴结转移无相关性(P=0.627,P=0.094和P=0.633)。结论:病理分级和hTERT蛋白表达可能是预测阴茎癌区域淋巴结转移的独立预后因素,可为阴茎癌临床进展的风险及预后的判断提供参考。
Objective： To study the correlation of the expressions of CD82 and hTERT and HPV infection with the clinical pathological features of penile cancer and identify their prognostic significance in the lymphatic metastasis of the disease.Methods： A total of 44 patients underwent partial or radical penectomy and lymph node dissection.The expressions of CD82 and hTERT were determined by immunohistochemistry,and HPV infection was detected by PCR.Results： The positive rates of CD82,hTERT,and HPV DNA in penile carcinoma were 47.7%,38.6% and 25.9%,respectively.The amplified HPV DNA was HPV-16.The pathological stage and hTERT expression were positively correlated with inguinal lymph node metastasis of penile cancer（P = 0.032,P = 0.041）,and so was the pathological stage with the expression of CD82（P = 0.045）,but neither the pathological stage,nor the expression of CD82 or the positive rate of HPV DNA showed any correlation with lymph node metastasis（P = 0.627,P = 0.094,P = 0.633）.Conclusion： The pathological grade and hTERT expression are independent prognostic factors for lymph node metastasis in penile carcinoma.These features help the prognosis and identification of the patient at the risk of nodal metastasis.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2011年第9期817-822,共6页
National Journal of Andrology
