摘要
目的探讨采用碳纳米管-硫堇(CNTs-THi)/苝四甲酸二酐衍生物膜(PTC-NH2)/纳米金(nano-Au)构建的新型的高灵敏癌胚抗原(CEA)免疫传感器的性能。方法以氧化铟锡(ITO)电极为基底,在其表面首先电沉积一层纳米金,然后依次修饰CNTs-Thi、PTC-NH2、nano-Au,固载癌胚抗原特异性抗体(anti-CEA)制得新型高灵敏癌胚抗原(CEA)免疫传感器。通过循环伏安法考察电极修饰过程的电化学特性。结果该免疫电极的峰电流响应与癌胚抗原的浓度在1.0～75.0 ng/mL的范围内保持良好的线性关系,相关系数为0.991 5,检测下限为0.5 ng/mL。结论利用该方法制备的免疫传感器具有灵敏度高、线性范围宽、检测下限低等优点。
Objective To explore the performance of a highly sensitive amperometric immunosensor for carcinoembryonic antigen（CEA） detection was made with carbon nanotubes-thionine（CNTs-Thi）,perylenetetracarboxylic dianhydride（PTC-NH2）and gold nanoparticle（nano-Au）.Methods Indium tin oxide（ITO） electrode was used as base,and on the surface carbon nanotubes-Thionine（CNTs-Thi） nanocomposite,3,4,9,10-perylenetetracarboxylic dianhydride（PTC-NH2）,nano-Au and anti-CEA was used.The CEA immunosensor was made.And then electrochemical property during the electrode modification process was observed by cyclic voltammetry（CV）.Results Under optimal conditions,the proposed immunosensor displayed a broader linear response to CEA,the working range was 1.0-75.0 ng/mL（r=0.9915） with a detection limit of 0.5 ng/mL.Conclusion The immunosensor made in this method exhibits high sensitivity,wider linear range,lower detection limit.
出处
《重庆医学》
CAS
CSCD
北大核心
2011年第27期2745-2746,2748,F0003,F0002,共5页
Chongqing medicine
关键词
纳米管
碳
癌胚抗原
免疫传感器
苝四甲酸二酐衍生物膜
nanotubes
carbon
carcinoembryonic antigen
immunosensor
3
4
9
10-perylenetetracarboxylic dianhydride
