摘要
目的:研究罗格列酮对体外培养小鼠前成骨细胞系MC3T3-E1生长与分化的影响。方法:以小鼠MC3T3-E1作为药物筛选的细胞模型,分别加入1、2、5、10μmo·lL-1罗格列酮干预,MTT法测定细胞活性并计算生长抑制率;流式细胞术检测细胞凋亡率;免疫组化法分析细胞中促凋亡因子Bax和抗凋亡因子Bcl-2蛋白的表达;测定细胞中分化指标碱性磷酸酶(ALP)的活性。设立只加入培养基处理的空白对照组。结果:与空白对照组比较,罗格列酮各浓度组生长抑制率、细胞凋亡率升高(P均<0.05),Bax表达增强、Bcl-2表达减弱、ALP活性下降(P均<0.05),且呈浓度依赖性。结论:罗格列酮可抑制MC3T3-E1细胞生长及分化,并诱导其凋亡;而Bax/Bcl-2可能参与凋亡的发生,并可能起关键调控作用。
OBJECTIVE： To study the effects of rosiglitazone on proliferation and differentiation of preosteoblast MC3T3-E1 cell of mice in vitro. METHODS： MC3T3-E1 cells were treated with 1, 2, 5, 10 μmol·L-1 rosiglitazone. Cell viability was measured by MTT to calculate growth inhibition rate. Cell apoptosis were inspected with flow cytometry, and the expressions of Bax and Bcl-2 were examined by immuno-chemical staining. And the activity of ALP was detected by alkaline phosphatase assay. MC3T3-E1 cells were only cultivated by culture media as blank control group. RESULTS： Compared with blank control group, the growth inhibition rate and apoptosis rate of rosiglitazone groups increased significantly （P〈0.05）, and Bax expression was attenuated while Bcl-2 expression and ALP activity decreased （P〈0.05） in dose-dependent manner. CONCLUSION： Rosiglitazone can inhibit the proliferation and differentiation of MC3T3-EI cell, promote apoptosis of MC3T3-E1 eell. Bax/Bcl-2 may be associated with the occurrence of apoptosis and play major regulation effect.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第37期3486-3488,共3页
China Pharmacy
