摘要
目的对天津市2010年的手足口病(HFMD)病例进行病原学分析,并对引起该市HFMD流行的肠道EV71型和柯萨奇病毒A组16型(Cox A16)进行分子生物学特征分析。方法利用Real-time PCR检测HFMD病例标本,用人横纹肌肉瘤(RD)细胞对Real-time PCR检测阳性的部分病例标本进行病毒分离,用RT-PCR法扩增VP1全基因,使用生物学软件进行序列和系统发生分析。结果共检测HFMD病例1766例,肠道病毒总检测阳性率为71.52%(1263/1766),其中EV71占43.94%,Cox A16占41.65%,其他EV占14.41%。15株EV71分离株与C4亚型的C4a分支代表株具有最高的核苷酸序列同源性,均归属于C4a分支;9株Cox A16分离株与B1亚型的代表株具有最高的核苷酸序列同源性,其中8株属于B1b分支,1株属于B1a分支。结论导致天津市2010年HFMD流行的EV71流行株为C4亚型中的C4a病毒株,Cox A16流行株为B1亚型的B1b病毒株。
Objective To understand the etiological characteristics of pathogens of hand foot and mouth disease（HFMD） and the molecular epidemiological characteristics of Enterovirus 71（EV71） and Coxasckievirus A16（Cox A16） in Tianjin in 2010.Methods Clinical specimens from HFMD patients were detected by using Real-time PCR.EV71 and Cox A16 strains were isolated from some positive specimens by using RD cell.VP1 genes of the isolates were amplified by using RT-PCR.Sequencing and phylogenetic analysis were carried out with biology software.Results A total of 1766 specimens were detected,the overall positive rate of enterovirus was 71.52%（1263/1766）.EV71 accounted for 43.94% and Cox A16 accounted for 41.65% of the total positive specimens,other enterovirus only accounted for 14.41%.Fifteen EV71 isolates had the highest homology with the representative strain of C4a cluster of the C4 subtype,9 Cox A16 isolates had the highest homology with the representative strain of B1 subtype,in which 8 belonged to B1b cluster,1 belonged to B1a cluster.Conclusion C4a strain of EV71 and B1b strain of Cox A16 were the major pathogens to cause HFMD in Tianjin in 2010.
出处
《疾病监测》
CAS
2011年第8期604-607,共4页
Disease Surveillance
