摘要
目的用RT-PCR检测中成药芎芍胶囊对心肌梗死大鼠缺血心肌血管新生作用。方法采用开胸结扎冠状动脉左前降支的方法建立心肌梗死模型,SD大鼠分为模型对照组和芎芍胶囊高、中、低剂量组,灌胃给药,连续给药6周;RT-PCR法检测缺血心肌EGF、VEGF、TGF-β1、PDGF-A、PDGF-BB等促血管生成因子的表达。结果其中芎芍胶囊低、中、高剂量组VEGF、EGF与模型对照组比较均有统计学意义,高剂量组对VEGF的促进作用较中、低剂量组有统计学意义,中剂量组对EGF的促进作用较高、低剂量组有统计学意义,对照组对TGF、PDGF促进作用较高、中、低剂量组有统计学意义。结论芎芍胶囊具有促进心肌梗死大鼠缺血心肌血管新生、提高心肌梗死大鼠心肌细胞VEGF、FGF、EGF蛋白的表达、具有保护心肌缺血性损伤的作用。
Objective:To detect the myocardial neovascularization effect of the Xiongshao Capsule on myocardial ischemia rats.Methods:The myocardial ischemia model was established by ligating the left anterior descending branch of the coronary artery.These SD rats were divided into the model control group,the Xiongshao Capsule high dose group,the Xiongshao Capsule middle dose group and the Xiongshao Capsule low dose group.After 6 weeks,the angiogenic factors in the angiogenesis such as EGF,VEGF,TGF-β1,PDGF-A and PDGF-BB.Results:There were some statistical differences in the VEGF,EGF,TGF and PDGF among 3 Xiongshao Capsule groups and the control group.Meanwhile,compared with the middle dose group and the low dose group,the increase of the high dose group was more significant in the VEGF.Compared with the high dose group and low dose group,the improvement of middle group was more obvious in EGF.Conclusion:Xiongshao capsule can improve the myocardial neovascularization and increase some proteins expressions including VEGF,FGF and EGF.It has the effect of myocardial ischemia injury.
出处
《中国中医急症》
2011年第9期1426-1427,共2页
Journal of Emergency in Traditional Chinese Medicine
基金
陈可冀中西医结合发展基金资助项目(CKJ20080432008J1004-06)
关键词
芎芍胶囊
心肌梗死
血管新生
RT-PCR
Xiongshao Capsule
Myocardial ischemia
Myocardial neovascularization
RT-PCR
