摘要
目的:建立镰形棘豆中鼠李柠檬素-3-O-β-新橙皮糖苷的含量测定方法,同时比较药材的不同部位经不同溶剂提取后所得含量。方法:采用HPLC法,色谱柱:Symmetry C18柱(4.6mm×250mm,5μm);流动相:1%乙酸水溶液-乙腈(79:21);流速:1.0mL/min;检测波长:350nm。结果:鼠李柠檬素-3-O-β-新橙皮糖苷在20-100μg/mL范围内线性关系良好(r=0.9995),平均回收率为99.49%,RSD=1.30%。溶剂对该化合物的提取率由高至低依次为甲醇,95%乙醇、水;药材叶中含量比根高。结论:本法简便、准确、重现性好,可用于该药材或相关制剂的质量控制;药材不同部位经不同溶剂提取所得含量差别较大。
Objective： To establish a HPLC method for determination of rhamnocitrin-3-O-β-neohesperidoside in Oxytropis falcate Bunge and to compare the content of rhamnocitrin-3-O-β-neohesperidoside in different parts of Oxytropis falcate Bunge with different extraction solvents.Methods：The HPLC method was used Chromatographic column： Symmetry C18（4.6mm×250mm,5μm） reversed-phase column;mobile phase： 1% acetic acid acid in water-acetonitrile（79：21）;flow rate： 1.0mL/min;detective wavelength： 350nm.Results： Rhamnocitrin-3-O-β-neohesperidoside was liner at the range of 20-100μg/mL（r=0.9995）,and its average recovery rate was 99.49%,RSD=1.30%.Conclusion： The method is simple,accurate and reproducible for the quality control of Oxytropis falcate Bunge.The contents of hamnocitrin-3-O-β-neohesperidoside are different in different parts of Oxytropis falcate Bunge with different extraction solvents.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第9期2068-2070,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
全军医药卫生"十一五"课题面上项目(No.06MB100)
南京军区医药卫生科研课题(No.06MA-95)~~
