摘要
【目的】探讨壳寡糖对甘蔗抗性相关次生代谢物及相关防御酶的影响。【方法】以甘蔗品种新台糖22号(ROC22)为材料,拔节初期对甘蔗叶片分别喷施25、50和75mg/L壳寡糖溶液,以喷施清水为对照(CK)。喷施1、2、3、4d后分别取+1叶测定壳寡糖诱导甘蔗叶多酚与防御酶(苯丙氨酸解氨酶、多酚氧化酶和过氧化物酶)活性的变化。【结果】50～75mg/L壳寡糖处理有利于甘蔗叶多酚积累;50mg/L壳寡糖处理2d后,苯丙氨酸解氨酶活性最大,为对照的1.8倍;50mg/L壳寡糖处理4d后,多酚氧化酶活性最高,为对照的1.7倍;75mg/L壳寡糖处理有利于提高甘蔗叶片过氧化物酶活性。【结论】50～75mg/L壳寡糖可以诱导甘蔗叶总多酚的积累及提高相关防御酶活性,这有利于提高甘蔗的整体抗性。
[Objective]The present study was carried out to explore the effect of chitosan oligosaccharide on secondary metabolites and some defense enzyme activities related to stress resistance in sugarcane. [Method]The leaves of sugarcane variety ROC22 were treated with 0 （CK）, 25, 50 and 75 mg/L chitosan oligosaccharide solution at the early elongation stage. The polyphenol content and the activities of defense enzymes, viz., phenylalanine ammonialyase （PAL）, polyphenol oxidase （PPO） and peroxidase （POD） were measured in ＋1 leaf of sugarcane at 1, 2, 3 and 4 d after spraying. [Result]50-75 mg/L chitosan oligosaccharide treatment was conducive to the accumulation of polyphenol in sugarcane leaf. The PAL activity reached to its maximum and was 1.78 times higher than the control after 2 d of 50 mg/L chitosan oligosaccharide treatment. The PPO activity reached to its maximum and was 1.7 times higher than the control after 4 d of 50 mg/L chitosan oligosaccharide treatment. The 75 mg/L chitosan oligosaccharide treatment was conducive to improve POD activity of sugarcane leaf. [Conclusion]It was found that 50-75 mg/L chitosan oligosaccharide treatment can improve the polyphenol accumulation and the activities of defense enzymes in sugarcane leaves, which is helpful for improving sugarcane resistance to various biotic and abiotic stresses.
出处
《南方农业学报》
CAS
CSCD
2011年第8期874-877,共4页
Journal of Southern Agriculture
基金
国家科技支撑计划项目(2007BAD30B03)
广西科技攻关重点项目(桂科攻0782004-3)
广西科技攻关项目(桂科能0815011)
广西自然科学基金项目(2010GXNSFA013101)
广西教育厅项目(200707MS034)
广西民族大学引进人才项目(2010QD022)
关键词
甘蔗
壳寡糖
多酚
苯丙氨酸解氨酶
多酚氧化酶
过氧化物酶
sugarcane
chitosan oligosaccharide
polypheols
phenylalanine ammonialyase （PAL）
polyphenol oxidase （PPO）
peroxidase （POD）
