摘要
研究了赤拟谷盗Tribolium castaneurn（Herbst）不同虫态（3-4龄幼虫、1-2龄蛹和羽化1天成虫）体内过氧化物酶（POD）、过氧化氢酶（CAT）、超氧歧化酶（SOD）活力在电子束辐照条件下的变化。结果表明,赤拟谷盗不同虫态的POD活力：羽化1 d成虫〉1-2龄蛹〉3-4龄幼虫;CAT活力：羽化1 d成虫〉1-2龄蛹〉3-4龄幼虫;SOD活力：3-4龄幼虫〉1-2龄蛹〉羽化1 d成虫。辐照处理后,保护酶活力随辐照剂量的增加呈先上升后下降的趋势,赤拟谷盗3种虫态POD活力均在500 Gy辐照剂量最高;3-4龄幼虫和1-2龄蛹CAT活力在420 Gy辐照剂量最高,羽化1 d成虫300 Gy最高;3-4龄幼虫和1-2龄蛹SOD活力在420 Gy辐照剂量最高,羽化1 d成虫500 Gy最高。本试验结果有助于阐明电子束辐照对储粮害虫的生理生化效应。
By electron beam irradiation,enzyme activity changes of peroxidase（POD）,catalase（CAT）,superoxide dismutase（SOD） of Tribolium castaneurn（Herbst） in different insects states（3-4th instar larvae,pupae age 1 day to 2 days and adult for 1 day eclosion） have been investigated.The results showed that POD activity of Tribolium castaneum in different states was in order： adult pupa larvae;CAT activity： adult pupa larvae;SOD activity： larvae pupa adult.After irradiation,the protection enzyme activity rose first,then fell with radiation dose increasing.POD of Tribolium castaneum in three states reached the highest vitality at 500 Gy,CAT of Tribolium castaneum 3-4th instar larvae,pupae age 1 day to 2 days and adult for 1 day eclosion reached the maximum at 420 Gy and 300Gy respectively,SOD of larvae and pupa up to the maximum at 420 Gy,and adult to the highest at 500 Gy.This test would contribute to illuminate the physiological and biochemical effect of electron beam irradiation on stored grain insects.
出处
《粮食储藏》
2011年第4期10-12,共3页
Grain Storage
基金
"十一五"国家科技支撑计划(项目编号:2006BAD08B02)
关键词
电子束
赤拟谷盗
过氧化物酶
过氧化氢酶
超氧歧化酶
electron beam
Tribolium castaneurn（Herbst）
peroxidase
catalase
superoxide dismutase
