摘要
目的研究高原地区上消化道疾病幽门螺杆菌(Helicobacter pylori,Hp)菌型。方法应用Hp尿素酶基因(ure)和细胞毒(cagA)基因引物对330例上消道疾病患者通过聚合酶链反应(PCR)技术,检测ure和cagA基因阳性检出率;用免疫印迹法检测血清ure和CagA抗体。结果 Hp菌株ure和cagA基因阳性检出率分别为90.0%和82.4%;Ure和CagA抗体阳性率分别为89.3%和63.6%;ure和cagA基因表达率分别为99.3%和77.2%。结论高原地区消化道疾病患者中HpcagA基因阳性率较高,CagA蛋白,CagA抗体(Ⅰ型Hp)的表达率亦相应增高。
Objective To study the biotypes of Helicobacter pylori（H.pylori） in plateau region of China. Methods DNA fragments of urease and cagA gene from 330 gastric biopsy specimens were amplified by PCR.Serum urease and CagA antibody of the patients with digestive disorders with H.pylori infection was examined by Western blot. Results The positive rates of urease and cagA genes were 90.0% and 82.4%, respectively. The positive rates of serum urease and CagA antibody were 89.3% and 63.6%, respectively. The expression rates of urease and cagA genes were 99.3% and 77.2%, respectively. Conclusions There is a higher positive rate of serum CagA antibody （Type I H.pylori） in plateau region of China.
出处
《现代消化及介入诊疗》
2011年第4期224-226,230,共4页
Modern Interventional Diagnosis and Treatment in Gastroenterology
关键词
上消化道疾病
幽门螺杆菌
菌型
尿素酶
细胞毒相关蛋白
Upper gastrointestinal diseases
Helicobacter pylori
biotypes
Urease
Cytotoxin associated protein
