摘要
利用SSR分子标记在分子水平探讨小麦种质资源抗麦长管蚜的遗传多样性,为高效节本型和环境友好型的抗蚜育种的研究和利用提供理论依据和技术支持。结果表明:在小麦的A、B和D 3组同源染色体组上的175对SSR分子标记鉴定出了有多态性的32对引物,其中在D同源组中小麦抗蚜性的遗传多样性较高,同时在21对染色体中7D染色体上遗传多样性较高,在7A染色体上的遗传多样性较低。并在此基础上用32对引物对14份小麦种质资源进行PCR分析,共得到255条清晰的条带,其中多态性条带244条,单条引物扩增出3～13条带,每条引物平均扩增出6.8条带,多态位点百分率为40%～100%。Nei’s基因多样性指数范围为0.3264～0.4243,Shannon信息指数范围0.5078～0.6154。聚类分析结果表明,14份种质资源按照小麦抗麦长管蚜特性分为3大类,并且具有相同抗蚜性的种质资源被划分为同一组。利用SSR分子标记技术准确分析了小麦种质资源抗蚜材料间的遗传多样性,并筛选出了用于鉴定小麦种质资源抗麦长管蚜的SSR分子标记和作为抗性亲本的优异种质资源。
The objective of this paper is to study the genetic diversity of wheat germplasm resistance to the grain aphid Sitobion avenae,providing theoretical basis and technical support for efficient breeding of environment-friendly wheat by SSR markers.A total of 32 pairs specific primers were selected from 175 pairs SSR primers distributing on A,B and D homologous chromosomes.The results showed that group D had higher genetic diversity than other groups.Among all the 21 pairs of chromosomes,genetic diversity was highest on chromosome 7D,and lowest on chromosomes 7A.A total of 255 bands were amplified with 32 pairs of primers among 14 wheat germplasm,of which 244 bands were polymorphic. Each pair of primers amplified 3 to 13 bands with an average of 6.8 bands.The percentage of polymorphic band was ranged from 40% to 100%.Nei＇s Genetic diversity and Shannon information index were in the range of 0.3264～0.4243 and 0.5078～0.6154,respectively.Cluster analysis showed that 14 wheat accessions could be divided into 3 groups by SSR,which were consistent with that of traditional classification,i.e.the germplasm resources with the same resistance were clustered in the same group.It is suggested that the genetic diversity of resistant to aphid in wheat germplasms could be efficiently assessed by SSR markers so that the SSR molecular markers could be effectively used in molecular breeding of new varieties resistant to aphid.
出处
《核农学报》
CAS
CSCD
北大核心
2011年第4期639-644,共6页
Journal of Nuclear Agricultural Sciences
基金
国家863计划重大专项(2009AA101102)
陕西省13115科技创新工程重大科技专项(2007ZDKG-020)
国家杨凌农业生物技术育种中心专项基金(99-1A)
西北农林科技大学拔尖人才支持计划项目
中德农业合作资助项目[2008/2009(04)]共同资助
