摘要
目的观察SCID鼠在人脐血干/祖细胞移植后免疫功能的变化,探讨移植后移植物抗宿主病(GVHD)的发生情况。方法采集足月健康胎儿新鲜脐血,以6%HES+NH4CL破红细胞液法分离人脐带血有核细胞。按随机原则将SCID鼠分为A、B、C三组,每组各8只:A组为脐血移植免疫功能重建、无肿瘤组;B组为脐血移植免疫功能重建、荷瘤组;C组为未重建免疫功能(免疫缺陷)、荷瘤组。A、B组分别经尾静脉程序移植(分5次),注入人脐血有核细胞(总量5×107),两组小鼠移植前都给予低剂量的环磷酰胺(150mg/kg),并连续使用甲基强的松龙1周(自20mg/kg逐渐减量至5mg/kg)作为预处理。C组为未移植对照组。观察各组小鼠免疫功能的变化及GVHD的发生情况。结果 (1)脐血移植后第3周即可在SCID小鼠外周血测得人IgG,第4周即出现人源性CD3+细胞并随着时间的延长其数值增加;(2)在观察期间SCID小鼠无腹泻、厌食、脱毛、皮疹、体重减轻、生长落后等表现,病理检查均未见明显GVHD的特征。结论人脐血程序移植加低剂量的环磷酰胺方案可替代照射预处理成功地在SCID鼠体内建立类似人类的细胞和体液免疫,并有效地预防GVHD的发生。
Objective to observe the SCID mice in the person umbilical cord blood stem/progenitor cells after transplantation immune function change,explore GVHD happened after transplantation.Methods Full-term health umbilical cord blood were collected and red cell was separated in 6% fetal fresh HES ＋ NH4CL by people with nuclei umbilical cord blood cells.SCID mice were divided into A,B and C three groups and there were 8 for each according to random principle.Group A was umbilical cord blood transplantation immunity function reconstruction,no tumor group,Group B was umbilical cord blood transplantation immunity function reconstruction,a tumor-burdened group.Group C was not rebuild immune function（immunodeficiency）,a tumor-burdened group.A,B group respectively by the tail vein program transplantation（points）,5 times into umbilical cord blood cells（who have nuclear total 5×107）,two groups of mice were given before transplantation low-dose cyclophosphamide（150mg/kg）,and the continual use methyl-prednisone week（since 20mg/kg gradually reduction to 5mg/kg） as A preprocessing.Group C for not transplant control group.Observe exp1 immune function in the changes and the occurrence of GVHD.Results After umbilical cord blood transplantation the third week can be measured in peripheral blood SCID mice,the first round of gad IgG that appear RenYuan sex CD3＋cells and with the extension of time its numerical increment.The observation period,SCID mice without diarrhea,anorexia,hair removal,rashes,weight loss,growth backward performance,there were no obvious pathological examination GVHD characteristics.Conclusion Human umbilical cord blood transplantation program with low-dose cyclophosphamide scheme alternative illuminate pretreatment successfully in SCID mice establish parallel human immune,and effectively prevent GVHD happening.
出处
《贵州医药》
CAS
2011年第7期582-586,共5页
Guizhou Medical Journal
