摘要
目的:复制肝前、肝内、复合型门脉高压犬模型,观察模型犬的舌底络脉变化。方法:制备3种门脉高压犬模型并行成模检测。肉眼观察舌下络脉形态和颜色,游标卡尺测量舌底根部络脉宽度,免疫组化检测舌底CD31的表达,透射电镜观察舌下络脉内皮细胞形态。结果:肝内和复合型组犬舌下血管较正常迂曲粗大,出现瘀点细络,颜色暗紫,络脉直径明显增粗,肝前型组仅络脉颜色变暗;与正常和假手术组比较,模型犬舌底CD31均显著增加,复合型组最明显;舌底血管壁全部或局部增厚,管腔变小,出现新的毛细血管,细胞膜界限不清,核损失甚或消失,复合型组程度最重,肝前型组改变较小。结论:门脉高压可引起动物舌底络脉宏观与微观改变,提示门静脉压力升高是导致其舌下络脉异常改变的一个重要因素。
Objective： To observe sublingual venae characteristics of three kinds of portal hypertension canine models. Methods： The portal hypertension models were made. The characteristics of sublingual venae were observed, and the diameters were quantitated by Slide calliper rule. The protein expression of CD31 were detected by immunohistochemical to represent capillary density. Endothelial cell morphology changes were observed with transmission electron microscope （TEM）. Results： The sublingual venae became circuity and thick with dark and violet color, and there were many petechia and microcollaterals around them in intrahepatic and composite portal hypertension canines compared with normal and sham animals. However, only color changes were abserved in prehepatic model. It was significantly increased expression of CD31 of animals in model group and the maximum expression was observed in composite model group. TEM results showed vessel wall of sublingual venae became thicker totally and partly, lumens became smaller, and new capillary appearanced, the edge of endothelial cells were blurred, and there were no nucleus almostly. As for the extent , the composite group was most serious, followed by the intrahepatic group, and prehepatic group was slightly. Conclusion： Micro or macro changes in sublingual venae of model animal may caused by portal hypertension. These demonstrated that the elevation of portal pressure is a crucial factor leading to abnormal of sublingual venae
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2011年第8期861-864,共4页
JOURNAL OF BASIC CHINESE MEDICINE
基金
国家自然科学基金资助项目(30772698)
上海市科委青年科技"启明星"人才计划资助项目(06QA14066)
关键词
门脉高压
舌下络脉特征
BEAGLE犬
portal hypertension
sublingual venae characteristics
Beagle dog
