摘要
目的:通过HPLC测定黄芩和半夏不同配伍比例后黄芩苷溶出量的变化,以探讨中药药性配伍的科学内涵。方法:采用Diamonsil C18(2)柱(4.6 mm×250 mm,5μm),乙腈-0.4%磷酸溶液(26∶74)为流动相,检测波长280 nm,流速1.0 mL.min-1,柱温30℃。建立黄芩与半夏配伍[以寒为主(9∶6);寒热各半(9∶9);以热为主(6∶9)]后黄芩苷的含量测定方法,并比较不同配伍后黄芩苷溶出量的大小。结果:黄芩苷质量浓度在0.010 25～0.102 5 g.L-1线性关系良好(r=0.999 9),平均回收率99.21%(n=9),RSD 2.0%;黄芩配伍半夏后[以寒为主(9∶6)、寒热各半(9∶9)、以热为主(6∶9)]和黄芩单煎后黄芩苷平均含量分别为64.018,63.6805,0.581,62.529 mg.g-1。结论:黄芩配伍半夏后黄芩苷的HPLC检测方法可靠、稳定、重复性好。不同比例配伍后黄芩苷的溶出量有较大差异,尤其是以热为主配伍(6∶9)对黄芩苷的溶出影响最大,溶出最低,体现了中药药性配伍中以半夏的温热之性抑制黄芩之寒凉之性的科学内涵。
Objective：Determination the leaching quantity of baicalin in different prop-ortional combinations of Scutellariae Radix and Prepared Pinellia tuber by HPLC,explore the scientific content about the compatibility of traditional Chinese medicine properties.Method：The HPLC separ-ation was performed on Diamonsil-C18 column（4.6 mm×250 mm,5 μm）,with a mixture acetonitrile-0.4% phosphate acid solution（26∶74） as mobile phase and detection wavelength of 280 nm.The flow rate was 1.0 mL·min-1 and the column temperature was 30 ℃.The experiments are 3 groups,the proportional combinations of Scutellariae Radix and Prepared Pinellia tuber are 9∶6,9∶9,6∶9 and comp-aring the leaching quantity of baicalin.Result：For baicalin,the linear rang was 0.010 25-0.102 5 g·L-1（r=0.999 9）,and the average recovery rate was 99.21%（n=9） with an RSD of 2.0%.Basis on the Scutellariae Radix equivalent,the Scutellariae Radix and Prepared Pinellia tuber are 9∶6,9∶9,6∶9 and single Scutellaria,the average content of baicalin are 64.018,63.680,50.581,62.529 mg·g-1.Conclusion：This HPLC reliable,stable and reproducible.The leaching quantity of baicalin has larger differences in different proportional combinations,especially the group of 6∶9 has the largest influence on leaching quantity,the leaching quantity is minimum.It reflects the scientific content about the properties of hot and warm of Prepared Pinellia tuber inhibition the properties of cool and cold of Scutellariae Radix.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第17期116-118,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
黄芩
法半夏
黄芩苷
药性配伍
Scutellariae Radix
Prepared Pinellia tuber
baicalin
compatibility
