摘要
[目的]研究甲氨喋呤-聚乳酸-羟基乙酸共聚物（MTX-PLGA）共聚物纳米囊的体外抑制效应。[方法]采用复乳法-溶剂挥发法制备MTX-PLGA共聚物纳米囊;用噻唑蓝（MTT）比色法检测不同浓度及不同时间MTX-PLGA共聚物纳米囊体外对人成骨肉瘤细胞株的抑制效应,以及与游离MTX对人骨肉瘤细胞株的抑制率相比较,并计算药物的半数抑制率IC50和对肿瘤细胞MG63的生长抑制率。[结果]不同浓度组在相同时间点的肿瘤细胞生长抑制率随药物浓度的增加而升高,不同浓度组间抑瘤率差异明显（P〈0.05）;在游离MTX组中,第3~6 d MTX-PL-GA组有显著的差异性（P〈0.05）。[结论]MTX-PLGA共聚物纳米囊可以作为MTX的有效缓释载体,实现化疗药物控释释放,延长化疗药物对肿瘤的有效作用时间。
[Objective]To study the antitumor effects of MTX loaded PLGA-nanoparticles on human osteosarcoma cell line in vitro.[Method]MTX loaded PLGA-nanoparticles were prepared by double emulsion method and solvent evaporation method.By MTT assay,the inhibitory effects of MTX-PLGA-NPs on human osteosarcoma cell line were detected at different concentrations and different times,and meanwile,calculated inhibitory rate compared to intracellular free MTX.The 50% inhibition concentrations values（IC50）and inhibitory rate of the MTX-PLGA-NPs against the MG63 cells were calculated.[Result]The inhibitory rate of tumor cell growth was improved as the drug concentration increaing at the same time,and the effects of the different groups had significant differences（P〈0.05）.Against intracellular free MTX,there were obviously different from the 3rd day to 6th day（P〈0.05）.[Conclusion] The MTX-PLGA-NPs could be used as an effective release carrier for MTX,be achieved to the release of the chemotherapeutic drug delivery,and prolonged the active time of the chemotherapeutic drugs.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2011年第16期1377-1380,共4页
Orthopedic Journal of China
基金
国家自然科学项目资助项目(编号:30600788)
上海市纳米专项基金资助项目(编号:05nm05014)
关键词
甲氨蝶呤
纳米囊
骨肉瘤
methotrexate
nanoparticles
osteosarcoma
