摘要
本试验以毒死蜱污染土壤为研究材料,利用降解菌DSP-A分别与高丹草、紫花苜蓿、多花黑麦草进行联合修复,探讨了植物-微生物联合修复毒死蜱污染土壤的效果,以及影响联合修复的因素,结果表明,植物-微生物联合修复的效果优于单一的植物修复及单一的微生物修复效果。与DSP-A菌群较合适的植物是高丹草,该组合对毒死蜱的降解率达到96.44%,其次是多花黑麦草。研究了微生物数量、植株密度以及土壤湿度对联合修复效果的影响,结果表明,DSP-A菌菌液稀释倍数越大,联合修复的效果越差。植株密度对联合修复的影响,主要表现为对植物根系生长的影响。植株密度越大,对生存环境的竞争越激烈,植物根系的生长越不好。除了紫花苜蓿外,高丹草和多花黑麦草根系的生长均受到影响。高丹草种植密度为12株/盆时,与DSP-A菌的联合修复效果最好,多花黑麦草则为10株/盆。土壤湿度是影响联合修复的重要因素,不仅影响植物的生长,对微生物的生长也有影响。土壤湿度过大,造成土壤的含氧量降低,不利于植物根系和好氧细菌的生长,从而影响土壤中农药的降解。土壤湿度过小,容易造成植株缺水,根系生长和微生物的生长。高丹草与DSP-A菌、多花黑麦草与DSP-A菌联合修复最适浇水量都为20 mL/d,紫花苜蓿与DSP-A菌联合修复最适浇水量都为15 mL/d。
Soil contamination by pesticides is an important problem all over the world. Bioremediation combining plants and microor- ganisms is increasingly recognized and applied to treat pesticide residues. This approach integrates the advantages of phytoremedi- ation and microbial remediation to accelerate degradation of the pesticides. Plant species Sorghum Hybrid, Lolium multi.forum and Medicago saliva, and a bacterium, DSP-A, were used to investigate plant-microorganism combined bioremediation of contaminated soil. The combined bioremediation effect of DSP-A with one of three plant species on chlorpyrifos in the contaminated soil was inves- tigated. The results showed that plant-microorganism combined bioremediation was better than phytoremediation or microbial remediation. Our study indicated that the best plant combined with DSP-A was Sorghum Hybrid, followed by Lolium multiforum. Factors affecting the combined remediation were studied. Microbial quantity, plant density and soil moisture played important roles in the combined bioremediation of chlorpyrifos .
出处
《生物安全学报》
2009年第2期81-87,共7页
Journal of biosafety
基金
福建省青年基金(2008F3021)
关键词
联合修复
毒死蜱
DSP-A菌
高丹草
紫花苜蓿
多花黑麦草
combined bioremediation
chlorpyrifos
DSP-A Strain
Sorghum Hybrid
Medicago sativa
Lolium multiforum
