摘要
目的对比分析重症婴幼儿龋(S-ECC)儿童及无龋儿童牙菌斑中变形链球菌(简称变链菌)的致龋性,为S-ECC的早期检测及预防奠定基础。方法按照NIH标准,将67名3～5岁儿童分为S-ECC组(n=35)、无龋组(n=32),采集牙面菌斑,经分离、鉴定获取纯化的变链菌株,接种至蔗糖浓度为20%的TYCSB液体培养基,检测其产酸能力、生长情况、合成水不溶性葡聚糖(WIG)的量以及黏附比值。结果 S-ECC组及无龋组中变链菌株的检出率分别为92.4%及62.5%,差别有统计学意义(P<0.05)。S-ECC组中变链菌致龋相关参数△pH值、A600值、合成WIG量及黏附比值显著高于无龋组(P<0.05)。结论 S-ECC患儿牙菌斑中变链菌的定植比例及临床分离菌株致龋性显著高于无龋儿童。
Objective To determine the cariogenicity of S.mutans in dental plaque of severe early childhood caries （S-ECC） and caries free children, and shed light on the early diagnosis and prevention of S-ECC. Methods According to the NIH criteria, 67 children with age ranging from 3 to 5 year-old were recruited and classified into S-ECC （n=35） and caries-free groups （CF, n=32）. Plaque samples were collected and cultured. Strains of S.mutans were identified, purified and cultured in TYCSB medium with 20% sucrose. Superuatant＇s pH value, was measured to evaluate acidgencity of S.muatans, and A600 to evaluate the propagating status. The quantities of water insoluble glucans （WIG） were measured by anthrone method. Turbidimetry was used to measure the adhesive ability. Results The detection ratios of S.mutans in S-ECC and CF group were 92.4% and 62.3%（P 〈 0.05）. △pH, A600, WIG and adhesion ratio of S mutans isolated from S-ECC group was statistically higher than that from CF group （P 〈 0.05）. Conclusion The detection ratio in dental plaque and cariogenicity of of S. mutans from S-ECC group was significantly higher than that from caries free group.
出处
《中华口腔医学研究杂志（电子版）》
CAS
2011年第4期15-18,共4页
Chinese Journal of Stomatological Research(Electronic Edition)
基金
广东省自然科学基金(8151008901000076)
广东省科技计划社会发展项目(00316971120302088)
关键词
婴幼儿龋
牙菌斑
变形链球菌
致龋性
Early childhood caries
Dental plaque
S.mutans
Cariogenicity
