摘要
目的；用胸腺嘧啶和５－溴脱氧尿嘧啶联合作用小鼠红白血病细胞，建立白血血病细胞同步化方法。方法：参照遗传学人外周血细胞同步化方法，细胞生长状态好的加入胸腺嘧啶，培养一定时间后加入５－溴脱氧尿嘧啶培养４．５－５小时后加入积水仙素，常规制备中期染色体标本，根据分裂相的多少了解同步化的效率。结果：经诱导后可获得大量中期染色体分裂相。
Objective:To establish a method of synchronizing leukemia cell with TdR and Brdu-induced MEL cell of mouse.Methods:Experimental methods of synchronization of human cell in genetics.Result:A lot of metaphase chromosomes were obtained after inducing.Conclusion:TdR and Brdu can synchronize mouse MEL cell culture better.
