摘要
目的 研究高渗氯化钠羟乙基淀粉40注射液（hypertomic sodiam chloride hydroxyethyl starch 40 injection，HSH）对小鼠局灶性脑缺血，再灌注损伤（ischemia／reperfusion injury，I/RI）的影响。方法30只昆明种小鼠，应用随机排列表进行随机分组，分成3组，每组10只：高渗氯化钠羟乙基淀粉40组（HSH组）、羟乙基淀粉（hydroxyetylstarch，HES）130／0．4组（HES组）、对照组（Control组）。于缺血后即刻经尾静脉注射不同药物：HSH组给予4ml／kHSH；HES组给予4ml／kg羟乙基淀粉130／0．4氯化钠注射液；Control组给予4ml／kg的生理盐水。缺血后12、24、72h行神经行为学评分；24h进行TYC染色，计算脑梗塞面积百分率；72h甲苯胺蓝染色，检测半暗带内存活神经元密度。结果缺血后12、24、72h的神经行为学评分，HSH组为10．0±2．0，11．4±1．8，10．4±1．8；HES组为12．8±1．8，10．2±1．9，10．6±2．7；Control组为12．0±2．1，10．2±2．4，10．0±2．5。缺血后24h的梗塞面积，HSH组为（34．0±7．8）％，HES组为（38．8±2．9）％，Control组为（35．6±6．5）％。缺血后72h的半暗带内存活神经元密度，HSH组为（264±57）个／mm2，HES组为（302±49）个／mm2，Control组为（288±69）个／mm2。3组小鼠的神经行为学评分、梗塞面积百分率以及半暗带内存活神经原元密度组间比较差异均无统计学意义（P〉0．05）。结论HSH对小鼠局灶性脑I／RI无改善作用。
Objective To investigate the effect of the hypertonic sodium chloride hydroxyethyl starch 40 injection on focal cerebral ischemia/reperfusion injury （I/RI） in mice. Methods Thirty KM mice were divided into three groups （n=10） randomly： hypertonic hydmxyethyl starch 40 sodium chloride group （group HSH）, hydroxyethyl starch 130/0.4 group （group HES）, control group （group control）. At onset of ischemic insult, different agents were injected via the tail vein instantly. Mice in group HSH received 4 ml/kg of hypertonic hydroxyethyl starch 40 sodium chloride injection. Mice in group HES received 4 ml/kg of hydroxyethyl starch 130/0.4 sodium chloride injection. Mice in group control received 4 ml/kg of 0.9% physiological saline injection. Neurological behavioral testing was performed at 12, 24 and 72 h after ischemia, respectively. Trc staining was performed at 24 h after ischemia, and the percentage of infarction area was calculated. Toluidine blue staining was performed at 72 h after ischemia and surviving neurons in the penumbra were counted. Results Neurological scoring at 12, 24 h and 72 h after ischemia： HSH group 10.0±2.0, 11.4±1.8, 10.4±1.8; HES group 12.8±1.8, 10.2±1.9, 10.6±2.7; Control group 12.0±2.1, 10.2±2.4, 10.0±2.5. The percentage of infarct area at 24 h after ischemia： HSH group （34.0±7.8）%; HES group （38.8±2.9）%; Control group （35.6±6.5）%. The density of surviving neurons in the penumbra at 72 h after ischemia： HSH group （264±57）/mm2; HES group （302±49）/mm2; Control group （288±69）/mm2. There were no difference in neurological deficit score, percentage of infarct areas and density of surviving neurons in the penumbra among three groups, respectively（P〉0.05 ）. Conclusion Hypertonic hydroxyethyl starch 40 sodium chloride injection has no etfect on focal cerebral I/RI in mice.
出处
《国际麻醉学与复苏杂志》
CAS
2011年第4期389-392,共4页
International Journal of Anesthesiology and Resuscitation
基金
国家自然科学基金资助项H（30772081）
教育部“新世纪优秀人才支持计划”（NECT-08-0436）
陕西省科技计划项目[2007K15-01（17）]
