摘要
目的 :观察体外分离培养的兔视网膜色素上皮 (RPE)细胞的形态学特点。方法 :用改良的 Stram m′s法分离培养兔 RPE细胞 ,每天在倒置显微镜下观察其生长状况 ,并进行超微结构的检查。结果 :离体培养的兔RPE细胞可呈圆形、梭形、六角形 ,并维持了体内细胞的许多生物学特性如 :顶端微绒毛、基底部内褶、细胞内的细胞器等 ,但细胞内色素成分随细胞的分裂逐渐减少。结论 :这一技术为在体外研究正常 RPE细胞的生理。
Objective:To observe the morphological feature of rabbit′s retinal pigment epithelium( RPE) cells in vitro.Methods:A modification of Stramm′s procedure was employed. A daily observation on the cell′s growth state and an ultrastructural examination were carried out under inverted microscope.Results:The cultured rabbit′s RPE cells occurred as round, fusiform,or hexagonal,and most of the normal morphological feature in vivo was maintained in vitro,including apical microvillus,basal infoldings,and intracellular organelles etal.But amount of pigment in the RPE cells was getting decreased along with the cell division.Conclusion:This technique makes things convenient for us to study the physiological,biochemical features of normal RPE cells in vitro.
出处
《山西医药杂志》
CAS
1999年第6期472-473,共2页
Shanxi Medical Journal
关键词
视网膜
芭素上皮细胞
分离
培养
超微结构够
兔
retinal pigment epithelium cells isolation/ culture ultrastructure rabbi
