摘要
目的 探讨代谢酶细胞色素Ｐ４５０ ２Ｅ１（ＣＹＰ２Ｅ１）和谷胱甘肽转硫酶Ｍ１（ＧＳＴＭ１） 基因多态性与胃癌的关系。方法 应用聚合酶链反应和限制性片段长度多态性技术对胃癌高发区福建省长乐市原发性胃癌病例９１ 例和正常对照９４ 例进行ＣＹＰ２Ｅ１ 与ＧＳＴＭ１ 基因型检测和分析。结果 胃癌病例与对照的ＣＹＰ２Ｅ１ 等位基因Ｃ１ 和Ｃ２ 频率差异有显著性（χ２ ＝４．９１，Ｐ＜０ ．０５） 。ＧＳＴＭ１ 基因缺失率在胃癌组和对照组分别是６１．５％ 和４５．７％ ，ＧＳＴＭ１基因缺失与胃癌易感性有关（ＯＲ＝１．９０，９５％ 可信限＝ １．０１～３．５６） 。携带ＣＹＰ２Ｅ１ 的Ｃ２／Ｃ２ 基因和ＧＳＴＭ１ 缺失基因型者长期摄入鱼露可增加胃癌发生的危险。结论 代谢ＣＹＰ２Ｅ１ 和ＧＳＴＭ１ 基因多态可能与胃癌的发生有关。
Objective To investigate the association of susceptibility to gastric cancer with gene polymorphism of metabolic enzymes cytochrome P450 2E1(CYP 2E1) and glutathione S transferase M1(GST M1).Methods The polymerase chain reaction restriction fragment length polymophism(PCR RFLP)was used to detect CYP 2E1 and GST M1 genotypes in cases with gastric cancer( n =91) and normal controls ( n =94)from a high risk area,Changle,Fujian.Results The frequencies of allele C1 and C2 of CYP 2E1 in gastric cancer cases were significantly higher than those in controls( χ 2=4.91,P<0.05 ).The gene deletion rate of GST M1 in gastric cancer cases and control group was 61.5% and 45.7%,respectively,with a statistical difference ( χ 2=4.61,P=0.0318 ).Conclusion The results suggest that CYP 2E1 and GST M1 gene polymorphisms may be associated with gastric cancer.\;
出处
《实用肿瘤杂志》
CAS
北大核心
1999年第6期330-333,共4页
Journal of Practical Oncology
基金
福建省科委基金!资助项目(K98031)
