摘要
目的和方法过量Ⅰ、Ⅲ型胶原的沉积是增生性瘢痕组织和瘢痕疙瘩形成的重要原因。采用两个自行构建的ＳＰ６体外转录系统，通过掺入同位素（ａ３２ＰＧＴＰ）进行放射自显影，探讨不同反义寡核苷酸对Ⅰ型胶原基因体外转录的抑制作用。结果和结论位于ＳＰ６转录起始位点区域的寡核苷酸能够有效抑制体外转录反应；而位于ＳＰ６转录起始位点下游约２００ｂｐ的寡核苷酸和作为对照的随机寡核苷酸对转录的抑制作用不明显。
Objective it is a very important factor that collagen type Ⅰ and type Ⅱaccumulates in excessive amount that causes theformation of keloids and hypertrophic scars. Method To understand the mechanism by which antisense oligodeoxynucleotide acts onin Vitro transcription of a1(I ) collagen gene, isotopes (a 32PGTP) was incorporated into two SP6 in vitro transcription systems.Results and conclusions Oligo-2 (at the transcription start region) could effectively inhibit in vitro transcription of pGEM3-Coll3and the control (random oligodeoxynucleotides) showed no inhibition. However, oligo-1 (at the transcription start region) obviouslyinhibited in the in vitro transcription of pGEM3-Coll4, while olig-2, which targeted the down stream region (about 200 bp) ofpromoter. showed no significant inhibition effect.
出处
《第一军医大学学报》
CSCD
1999年第5期426-428,共3页
Journal of First Military Medical University
关键词
Ⅰ型胶原基因
反义寡核苷酸
体外转录
瘢痕
a1(I ) collagen gene
antisense oligodeoxynucleotide
in vitro transcription
