摘要
目的:研究不同产地、不同成熟期淫羊藿叶中淫羊藿苷和朝藿定C的含量变化。方法:采用Elite SinoChromODS-AP色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈(A)-水(B),梯度洗脱(0～8 min,27%A;8～30min,27%～29%A),流速为1mL.min-1,检测波长为270nm。结果:不同产地粗毛淫羊藿的淫羊藿苷、朝藿定C的含量变化较大,依次分别为0.1%～0.44%、0.76%～2.59%;不同成熟期淫羊藿的淫羊藿苷和朝藿定C在嫩叶中的含量均高于老叶数倍。结论:试验结果表明:产地对粗毛淫羊藿中淫羊藿苷和朝藿定C含量影响较大;不同成熟期淫羊藿叶中淫羊藿苷和朝藿定C的含量变化可达2～11倍。
Objective：To study the content variation of 2 mainly components in leaf of Epimedium of different areas and maturity stage.Methods：The samples were separated on Elite SinoChrom ODS-AP column（250 mm×4.6 mm,5μm） by gradient elution with acetonitrile（A）-water（B）（08 min,27 % A;830 min,27 %29 % A） as the mobile phase at a flow rate of 1 mL·min-1 and the detecting wavelength was 270 nm.Results：The contents of icariin and epimedin C in Epimedium acuminatum of different areas were 0.1%-0.44% and 0.76%-2.59% respectively.The contents of icariin and epimedin C in Epimedium acuminatum of tender leaf were several times higher than the old’s.Conclusion：The variation of the contents of icariin and epimedin C were obvious in Epimedium acuminatum of different areas.The contents of icariin and epimedin C in different maturity stage of leaf of Epimedium were changed from 2 times to 11 times.
出处
《辽宁中医杂志》
CAS
北大核心
2011年第8期1627-1629,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
贵州省中药现代化科技产业研究开发专项(黔科合社字[2008]5029号)
国家中医药管理局重点学科(黔科合计2字[2009]4008号)
贵州省科技创新人才团队黔科合人才团队([2008]4008号)
