摘要
分析了兔骨骼肌肌质网磷脂的组成及摩尔分数，并用气相色谱法对两种主要磷脂的脂肪酸组成及摩尔分数进行了测定。
Phospholipids were extracted from the sarcoplasmic reticulum of rabbit skeletal muscle, and separated by high performance thin layer chromatography. The mole fraction of individual phospholipids were determined by assaying phosphorus of each band. Main phospholipids were scraped and extracted, and subjec ted to esterification by alkaline methanol, and then the composition and content of fatty acids were determined by gas chromatography. The results show that the main phospholipid components were PC(phosphatidylcholine) and PE(phosphatidylethanolamine). They account for 66.5% and 15.5%, respectively. The main fatty acid in PC is palmitic acid and those in PE are stearic acid and arachidic acid.
出处
《色谱》
CAS
CSCD
北大核心
1999年第6期547-549,共3页
Chinese Journal of Chromatography
基金
辽宁省教委1997年资助
关键词
气相色谱
肌质网
磷脂
脂肪酸
测定
兔
骨骼肌
gas chromatography
sarcoplasmic reticulum
phospholipids
fatty acids
