摘要
采用高效液相色谱法,对湘玉竹进行了指纹图谱分析。采用Welchrom-C8(4.6 mm×250 mm,5μm)色谱柱,0.2%磷酸水-乙腈为流动相,梯度洗脱。结果表明,建立湘玉竹HPLC指纹图谱,标定10个共有指纹峰。该方法准确、可靠、有效,可为评价湘玉竹提供科学依据。
Using high performance liquid chromatographic（HPLC） method to conduct the analysis of fingerprint for Polygonatum odoratum.The method use Welchrom-C8（4.6 mm×250 mm,5 μm） chromatographic column,0.2% phosphoric acid-acetonitrile solution as mobile phase with a gradient elution to establish the HPLC fingerprint of Polygonatum odoratum,in which 10 common peaks in fingerprint were demarcated.This method is accurate,reliable and effective which provides an important basis for evaluating Polygonatum odoratum.
出处
《湖南农业科学》
2011年第7期137-138,共2页
Hunan Agricultural Sciences
