摘要
反义技术是根据碱基互补原理，利用与靶ＤＮＡ 或靶ＲＮＡ 互补的短链寡聚核苷酸片段抑制基因表达的方法。反义技术，在理论上作为一种完美的、特异性的方法阻断基因的表达，在短短的二十年里取得了迅速发展。本文将对反义技术、反义技术存在的问题及可能的解决方法、以及当前反义技术在医学中的应用进行阐述，本文还将对反义技术未来的发展趋势进行讨论。
Antisense technique,developed on the principle of base complementation,is a method using short strand oligonucleotide fragments complementary to target DNAs or target RANs for inhibition of gene expression.As a theoretically perfect and specific method to block the expression of gene,it has been rapidly developed over the last twenty years.This review expatiates on its techniques,its problem and possible way of problem solving,and its recent application in medicine.Also this review discusses the trends of its further development.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
1999年第4期516-519,共4页
Journal of Biomedical Engineering
