摘要
为测定3,5-二叔丁基-4羟基-苄基甲醚的抑菌活性,分别采用生长速率法、悬滴法测定了该物质对6种植物病原真菌菌丝生长及孢子萌发的抑制作用。结果表明:该物质抑制6种病菌菌丝生长的EC50在186.48~711.78 mg.L-1之间,抑制孢子萌发的EC50在171.93~337.85 mg.L-1之间,且抑制孢子萌发的EC50普遍低于抑制菌丝的EC50。其中对番茄灰霉病菌的抑制作用最强,对菌丝和孢子萌发的EC50分别为186.48和171.93 mg.L-1。同时,比较了3,5-二叔丁基-4羟基-苄基甲醚与多菌灵、甲基托布津对番茄早疫及梨黑斑病菌的抑制作用,结果表明:该化合物抑制2种病菌菌丝生长的EC50虽然稍高于甲基托布津对病菌的EC50,但均低于多菌灵,表明该物质具有一定的开发应用前景。
In order to measure the antibacterial activity of 3,5-di-tert-butyl-4-hydroxybenzyl methyl ether,the inhibition of mycelia growth and spore germination for six plant pathogenic fungi were measured by the growth rate and pendent drop methods.The results showed that: EC50 of the substance inhibiting mycelia growth of six kinds of plant pathogenic fungi were between 186.48 to 711.78 mg·L-1,EC50 of inhibiting spore germination were between 171.93 to 337.85 mg·L-1,and EC50 of inhibiting spore germination was generally lower than that of mycelia growth.The effect of which was most obvious against Botrytis cinerea,EC50 for mycelia and spore germination were 186.48 and 171.93 mg·L-1,respectively.Meanwhile,the inhibition of mycelia growth of 3,5-di-tert-butyl-4-hydroxybenzyl methyl ether and Carbendazim,Thiophanate-methyl to Alternaria solani and Alternaria alternata were compared.The results showed that: EC50 for mycelia growth of the compound was higher than that of Thiophanate-methyl,but lower than that of Carbendazim,suggesting that the compound had a certain development prospect.
出处
《山西农业大学学报(自然科学版)》
CAS
2011年第3期209-212,共4页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
山西省科技攻关项目(20100311038
20110311013-1)
山西省留学归国人员基金(2009043)