摘要
在碱性条件下,降解青霉素G并与6-氨基己酸反应,首次成功合成了纯度较高的半抗原。采用活化酯法与匙孔血蓝蛋白(KLH)偶联,成功制备了具有青霉素G结构特征的人工抗原。通过免疫获得了高效价、高特异性的多克隆抗体,抗血清效价达1∶240000。绘制了青霉素G酶联免疫直接竞争法的标准曲线,检测限可达0.04μg/L,灵敏度0.37μg/L。
Penicillin G was degraded in alkaline solution to react with 6-aminocaproic acid（EACA） . High purity of BPO-EACA was firstly prepared. Artificial antigen was produced by coupling the hapten to KLH with an active ester method. The anti-Penicillin polyclonal antiserum with high affinity and specificity was obtained which had a titer of 1∶ 240 000. The stand curve of the direct competitive ELISA for penicillin G was developed. The detection limit and sensitivity were 0.04 μg/L and 0.37 μg/L separately.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2011年第4期168-171,共4页
Journal of Chinese Institute Of Food Science and Technology
基金
天津市应用基础及前沿技术研究计划重点项目(09JCZDJC19400)
关键词
青霉素G
人工抗原
酶联免疫
penicillin G
artificial antigen
enzyme-linked immunosorbent assay
