摘要
共焦扫描光学显微（ＣＳＯＭ）系统有透射式和反射式、相干光和非相干光荧光等类型。ＣＳＯＭ 系统的平面分辨率和纵深分辨率依赖于放置在光电探测器前面的空间滤波器的孔径大小。根据不同孔径计算反射式荧光ＣＳＯＭ 系统的非相干光学传递函数。
Confocal scanning optical microscopy (CSOM) includes transmission type and reflection type as well as coherent incident light type and incoherent fluorescent light type.The resolutions of CSOM system in both transverse direction and axial direction depend on the pinhole radius of the space filter in front of the photodetector.The incoherent optical transfer functions of the reflective fluorescent CSOM system are calculated for different radii and the dependence of its imaging property on the pinhole radius is illustrated.
出处
《南京邮电学院学报》
1999年第3期31-34,共4页
Journal of Nanjing University of Posts and Telecommunications(Natural Science)
关键词
共焦扫描
光学传递函数
显微成像系统
Confocal scanning
Optical transfer functions
Incoherent optical transfer functions
