摘要
以甘蔗(新台糖22号)茎尖脱毒组培苗为材料,利用间歇浸没式生物反应器(TIBs)进行组培快繁的技术体系研究.结果表明:TIBs系统可使甘蔗组织培养一代增殖40倍以上,远远高于传统方法;TIBs系统以第3代继代的材料为宜;10～15株/瓶的接种密度最有利于组培苗的增殖;激素选择与传统方法相似,0.5～1.0 mg/L 6-BA适合甘蔗组织的增殖,4 mg/L NAA有利于根的诱导;浸没间歇频率以浸没1 min间歇3 h处理的增殖和生长表现比较优异,而6 h时则有利于根的诱导和根的生长.
A new protocol was established for sugarcane propagation in a temporary immersion bioreactors system（TIBs）.The results showed that TIBs increased a multiplication rate to more than 40 times in one subculture,the most suitable starting materials were the third sub-cultured plantlets and the most suitable density inoculums were 10-15 plantlets per liter in TIBs.0.5-1.0 mg/L 6-BA was suitable for multiplication and 4 mg/L NAA was suitable for rooting.Temporary frequency of 1 min per 3 h was suitable for multiplication,1 min per 6 h for rooting.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2011年第3期37-41,共5页
Journal of South China Agricultural University
基金
国家科技支撑计划项目(2007BAD30B03)
科技部国际合作项目(2008DFA30600,2009DFA30820
广西自然科学基金(桂科自0832087)
关键词
间歇浸没式生物反应器
甘蔗
组培快繁
temporary immersion bioreactors system
sugarcane
rapid propagation
