摘要
目的研究多囊卵巢综合征(polycystic ovary syndrome,PCOS)患者腹部脂肪组织中c-Jun氨基端激酶1(JNK1)、肿瘤坏死因子受体相关因子2(TRAF2)的表达,探讨PCOS发生胰岛素抵抗(insulin resistance IR)的分子机制。方法分别选择PCOS组(肥胖13例和非肥胖15例)、对照组(肥胖、非肥胖各20例)。放射免疫法检测各实验组血清黄体生成激素(1uteinizinghormone LH)、卵泡刺激素(follicle stimulating hormone FSH)、睾酮(testosterone T)、血清空腹胰岛素(fasting insulin FINS)的水平;用葡萄糖氧化酶法测定空腹血糖(fasting plasma glucose FPG);采用稳态模型胰岛素抵抗(Homeostasis model assessment-insulin re-sistance HOMA-IR)模型计算胰岛素抵抗指数(HOMA-IR);采用逆转录聚合酶链式反应(RT-PCR)方法检测PCOS组和对照组患者腹部脂肪组织中JNK1、TRAF2 mRNA的表达情况。结果 (1)所有PCOS组LH、LH/FSH、T、FINS、HOMA-IR均高于对照组(均P<0.05);(2)JNK1、TRAF2mRNA的相对表达量在PCOS肥胖组高于对照肥胖组(均P<0.01),在PCOS非肥胖组高于对照非肥胖组(均P<0.01),且PCOS肥胖组较PCOS非肥胖组高(均P<0.01)。结论 JNK1、TRAF2在多囊卵巢综合征中的高表达可能与PCOS发病机制存在一定关系。
Objective To research the expression of c-Jun N-terminal kinase1 and tumor necrsis factor receptor associated factor 2 in adipose tissue of women with polycystic ovary syndrome,to discuss the molecular mechanisms of Insulin resistance in PCOS.Methods PCOS group（obese 13cases、non-obese 15 cases）,Control group（obese,non-obese 20 cases）.Radioimmunoassay was used to measure each groups serum luteinizing hormone（LH）,follicle stimulating hormone（FSH）,testosterone（T）,serum fasting insulin（FINS） level;with glucose enzymatic measurement of fasting blood glucose（FPG）;with homeostasis model assessment（HOMA） model insulin resistance index（HOMA-IR）;reverse transcription polymerase chain reaction（RT-PCR） was used to measure JNK1,TRAF2 mRNA expression in adipose tissue of PCOS group and control group patients.Results All PCOS groups LH,LH / FSH,T,FINS,HOMA-IR were higher than the control group（P0.05）;（2） the expression of JNK1,TRAF2 in obese PCOS was higher than the obese control group（P0.01）,the non-obese PCOS group is still higher than the non-obese control group（P0.01）,obese PCOS group is higher than non-obese PCOS group（P0.05）.Conclusion JNK1,TRAF2 is highly expressed in PCOS may imply a possible role for JNK1 and TRAF2 in the pathogenesis of PCOS.
出处
《江西医药》
CAS
2011年第6期507-511,共5页
Jiangxi Medical Journal
基金
江西省卫生厅课题编号(20083104)
