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异龄蒙古羊肌肉组织中差异表达基因的研究 被引量：1

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摘要 本研究旨在筛选不同年龄蒙古羊肌肉组织中的差异表达基因,以3日龄、5年龄蒙古羊相同部位的肌肉组织为实验素材,采用mRNA差异显示技术检测蒙古羊肌肉组织中表达差异的基因,实验获得9条差异表达条带;对其中1条差异表达显著的片段进行测序,获的一段长度为241 bp的mRNA序列,与牛的TMP1基因同源性为97%,实时定量PCR技术进一步检测发现TPM1在3日龄羊肌肉中的相对表达量为5年龄羊中的2.675倍。
作者 杨杰 王峰 张焱如 周欢敏
机构地区 内蒙古农业大学动物遗传育种重点实验室
出处 《上海畜牧兽医通讯》 2011年第4期6-8,共3页 Shanghai Journal of Animal Husbandry and Veterinary Medicine
基金 内蒙古自治区重大科技专项"草原牛羊特色优异基因的发掘及育种新技术研究与应用"
关键词 蒙古羊 MRNA差异显示 原肌球蛋白基因1
分类号 S826 [农业科学—畜牧学]
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参考文献10

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同被引文献17

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上海畜牧兽医通讯
2011年 第4期

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