期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RGD肽介导的MR分子探针在体外结直肠癌细胞的显像及对其生物学行为的影响 被引量：2

The Vitro Imaging of RGD Peptide-Mediated MR Molecular Probes and Its Influence in Colorectal Cancer Cells
原文传递
导出
摘要 目的:探讨RGD肽介导的MR分子探针在体外结直肠癌细胞的MRI显像及对其生物学行为的影响方法:利用纳米技术构建靶向RGD荧光纳米MR探针,利用荧光倒置相差显微镜观察该探针与LOVO细胞结合情况;体外磁共振成像(MRI)显像;利用细胞克隆实验测其增殖活性;流式细胞术检测其细胞周期、凋亡。结果:荧光相差显微镜示该RGD肽介导的MR分子探针能特异性与LOVO细胞结合;体外MRI成像示靶向RGD组T_1信号强度高于非靶向组及对照组(P<0.05);该探针作用24h后的LOVO细胞增殖活性降低,细胞分裂周期发生变化,阻滞在S+G_2M期的细胞比例上升,细胞凋亡率与其他两组相比有显著增加(P<0.05)结论:该RGD肽介导的MR分子探针能与结直肠癌LOVO细胞靶向结合,能增强MRI的显像效果。 Objective： To investigate the effect of the RGD peptide-mediated MR molecular probes on the MRI application of LOVO cells in vitro and the biological behavior. Methods： The nano-technology constructed RGD peptide-mediated MR molecular probes were used. The binging situation was detected by the fluorescence phase contrast microscope, MRI test T~ signal intensity. The cell colon and Flow cytometry were used to analyze the proliferative situation. Result： The RGD peptide-mediated MR molecular probes can binding with the LOVO cell specificity. The signal intensity of targeted probe group was higher than that in others two groups（P〈0.05）. The cell proliferation activity of targeted probe group decreased. RGD peptide-mediated MR molecular probes can block G2＋S period of LOVO cell and increased the apoptosis rate （P〈0.05）. Conclusions： The RGD peptide-mediated MR molecular probes can specifically adsorbed with colorectal cancer LOVO cells, and it can target enhanced the MRI imaging results, and may have some inhibited effect on tumor cells.
作者 宋歌 刘峰君 程英升
机构地区 苏州大学 同济大学附属第十人民医院影像临床医学中心
出处 《现代生物医学进展》 CAS 2011年第15期2810-2813,2805,共5页 Progress in Modern Biomedicine
基金 国家自然科学基金(51003078) 上海市科委基础研究重点项目(10JC1412900)
关键词 RGD肽 MR分子探针 结直肠癌 分子影像 RGD peptide MR molecular probes Colorectal cancer Molecular imaging
分类号 R735.3 [医药卫生—肿瘤]
  • 相关文献

参考文献12

  • 1Sicinski P, Donaher JL, Geng Y, et al. Cyclin D2 is an FSH-responsive gene involved in gonadal cell proiferation and oncogenesis [J].Nature, 1996,384(6608):470-474.
  • 2Alam H, Maizels ET, Park Y, et al. Follicle-stimulating hormone activation of hypoxia-inducible factor-1 by the phosphatidylinositol 3-kinase/AKT/Ras homolog enriched in brain (Rheb)/mammalian target of rapamycin (mTOR) pathway is necessary for induction of select protein markers of follicular differentiation [J]. J Biol Chem, 2004, 279(19): 19431 - 19440.
  • 3Meroni SB, Riera MF, Pellizzari EH, et al. FSH activates phosphatidy- linositol 3-kinase/protein kinase B signaling pathway in 20-day-old Sertoli cells independently of IGF-I [J].J Endocrinol, 2004, 180 (2): 257-265.
  • 4Choi JH, Choi KC, Auersperg N, et al. Gonadotropins upregulate the epidermal growth factor receptor through activation of mitogen-acti- vated protein kinases and phosphatidyl-inositol-3-kinase in human ovarian surface epithelial cells [J].Endocr Relat Cancer,2005, 12(2): 407-421.
  • 5Syed V, Ulinski G, Mok SC, et al. Reproductive hormone-induced,STAT3-mediated interleukin 6 action in normal and malignant hman ovarian surface epithelial cell [J].J Natl Cancer Inst,2002,94 (8): 617-629.
  • 6Purbey PK, Singh S, Kumar PP, et al. PDZ domain-mediated dimeriza- tion and homeodomain-directed specificity are required for high-affini- ty DNA binding by SATB1 [J].Nucleic Acids Res, 2008, 36 (7): 2107-2122.
  • 7Purbey PK, Singh S, Notani D, et al. Acetylation-dependent interaction of SATB1 and CtBP1 mediates transcriptional repression by SATB1 [J]. Mol Cell Biol, 2009, 29(5):1321-1337.
  • 8Seo J, Lozano MM, Dudley JP. Nuclear matrix binding regulates SATBl-mediated transcriptional repression [.1]. J Biol Chem, 2005, 280(26):24600-24609.
  • 9Cai S, Han H J, Kohwi-Shigematsu T. Tissue-specific nuclear architect- ure and gene expression regulated by SATB1 [J]. Nat Genet, 2003,34 (1):42-51.
  • 10Alvarez JD, Yasui D H, Niida H, et al. The MAR-binding protein SATB 1 orchestrates temporal and spatial expression of multiplegene- s during T-cell development [J].Genes Dev, 2000, 14(5):521-535.

二级参考文献12

  • 1Jemal A, Siegel R, Ward E, et al. Cancer Statistics, 2009 [J]. CA Cancer J Clin,2009, 59(4):225-249.
  • 2Dickinson LA, Joh T, Kohwi Y, et al. A tissue-specific MAR/SAR i DNA-binding protein with unusual binding site recognition [J].Cell, 1992,70(4):631-645.
  • 3Purbey PK, .Singh S, Kumar PP, et al. PDZ domain-mediated dimerization and homeodomain-directed specificity are required for high-affinity DNA binding by SATB 1 [J].Nucleic Acids Res, 2008, 36 (7): 2107-2122.
  • 4Purbey PK, Singh S, Notani D, et al. Acetylation-dependent interaction of SATB 1 and CtBP 1 mediates transcriptional repression by SATB 1 [J]. Mol Cell Biol, 2009, 29(5): 1321-1337.
  • 5Seo J, Lozano MM, Dudley JP. Nuclear matrix binding regulates SATBl-mediated transcriptional repression [J]. J Biol Chem, 2005, 280(26):24600-24609.
  • 6Cai S, Han HJ, Kohwi-Shigematsu T. Tissue-specific nuclear architecture and gene expression regulated by SATB1 [J]. Nat Genet, 2003,34 (1):42-51.
  • 7Alvarez JD, Yasui D H, Niida H, et al. The ,MAR-binding protein SATB 1 orchestrates temporal and spatial expression of multiplegenes during T-cell development [J].Genes Dev, 2000, 14(5):521-535.
  • 8Nie H, Yao X, Maika SD, et al. SATB1 is required for CD8 coreceptor reversal[J]. Mol Immunol, 2008, 46(1): 207-211.
  • 9Cai S, Lee CC, Kohwi-Shigematsu T. SATB1 packages densely looped, transcriptionally active chromatin for coordinated expression of cytokine genes[J].Nat Genet,2006,38( 11): 1278-1288.
  • 10Zhang J, Ma C, Han X, et al. The bcl-2 major breakpoint region (mbr) . possesses transcriptional regulatory function [J].Gene, 2006,379(9): 127-131.

共引文献3

同被引文献24

引证文献2

现代生物医学进展
2011年 第15期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部