摘要
The determination method of L-phenylalartine （LPA） by fluorescence quenching was developed. The assay was based on the combination of the cucurbit[7]uril （CB [7]） with palmatine hydrochloride （PAL） reaction. In the presence of CB [7], the fluorescence of PAL was quenched by LPA which can be employed to detect LPA. Under the optimal conditions, a linear range 3.63 ×10^-8- 9.68 ×10^-6 mol/L and a detection limit 1.27 ×10^-8 mol/L of LPA were obtained. The relative standard deviation （R.S.D） was 1.8% obtained from a series of 11 standards each containing 6.05 ×10^-6 mol/L of LPA. This paper also discusses the mechanism of fluorescence indicator probe.
The determination method of L-phenylalartine （LPA） by fluorescence quenching was developed. The assay was based on the combination of the cucurbit[7]uril （CB [7]） with palmatine hydrochloride （PAL） reaction. In the presence of CB [7], the fluorescence of PAL was quenched by LPA which can be employed to detect LPA. Under the optimal conditions, a linear range 3.63 ×10^-8- 9.68 ×10^-6 mol/L and a detection limit 1.27 ×10^-8 mol/L of LPA were obtained. The relative standard deviation （R.S.D） was 1.8% obtained from a series of 11 standards each containing 6.05 ×10^-6 mol/L of LPA. This paper also discusses the mechanism of fluorescence indicator probe.
基金
supported by the Research Fund for the Doctoral Program of Higher Education of China(No. 20091404110001)
the foundation of Shanxi Normal University(No.ZR1007)
