摘要
通过刚果红染色法初筛,结合产酶活性测定复筛,从奶牛、山羊瘤胃内容物中分离到一株纤维素高效降解菌NL-3,克隆了该菌的18S rDNA序列,并以其同源性为基础构建了相关属种的系统发育树。结果表明,18S rDNA序列全长504 bp,Blast显示该菌株与青霉菌属同源;NL-3菌株在进化关系上也与青霉菌属(Penicillium)聚成一族。特别是与Penicillium decumbensstrain JU-A10的同源性最高,序列相似性达99%。结合菌株形态学及18S rDNA基因序列分析结果对菌株进行鉴定,初步鉴定为青霉属的斜卧青霉(Penicillium decumbens)。
A fungal strain named as NL- 3 that could degrade cellulose was isolated from the rumen of cows and sheep. The 18S rDNA gene sequence for strain NL- 3 was amplified and sequenced. The results showed that the complete 18S rDNA gene sequence was 504 bp. A phylogenetic tree was constructed by comparing the 18S rDNA sequence with other relative fungus species in the GenBank database. The strain NL -3 and Penicillium decumbens strain JU -A10 constituted a branch with the similarity value as 99%. According to the morphological characteristics and phylogenetic analysis, the strain NL- 3 was identified as Penicillium decumbens.
出处
《山东农业科学》
2011年第7期5-8,共4页
Shandong Agricultural Sciences
基金
山东省肉品质量安全控制工程技术研究中心组建项目资助
关键词
纤维素降解真菌
鉴定
系统发育分析
Cellulose - degrading fungus
Identification
Phylogenetic analysis
