摘要
目的观察删除CD4+CD25+T细胞对OVA免疫小鼠体液免疫应答的影响。方法小鼠腹腔注射抗CD25单克隆抗体,分别于3天、10天、27天采血流式检测外周血中CD4+CD25+T细胞的比例;注射抗CD25单克隆抗体3天后,OVA加铝佐剂免疫未删除和删除CD4+CD25+T细胞小鼠,7天后加强免疫一次,分别于初次免疫后7天,加强免疫后14天采血制备血清,ELISA法检测血清中OVA特异性IgE和IgG1的浓度。结果注射抗CD25单克隆抗体3天和10天,外周血中无CD4+CD25+T细胞;27天后CD4+CD25+T细胞的比例部分恢复。初次免疫7天后,删除CD4+CD25+T细胞小鼠总IgE、OVA特异性IgE和IgG1浓度较未删除组升高;加强免疫14天后,删除CD4+CD25+T细胞小鼠OVA特异性IgE较未删除组升高。结论删除CD4+CD25+T细胞能够影响小鼠OVA特异性体液免疫应答。
Objective To evaluate whether depletion of CD4 ＋ CD25 ＋ T cells leads to increased humoral immune response of mice immunized with OVA. Methods Groups of mice received one i.p. injection of anti-CD25 mAb, 3、10、27 days later, blood was harvested and the percentage of CD4 ＋ CD25 ＋ T cells was determined by FACS. 3 days after injection of anti-CD25 mAb, depleted and un-deplleted CD4 ＋ CD25 ＋ T cells mice were immunized with OVA ＋ ALUM, and boosted 7 days later. Blood was harvested from mice 7 days after prime and 14 days after boost, and OVA specific IgE and IgG1 in sera were determined by ELISA. Results 3 days and 10 days after injection of anti-CD25 mAb, there were no CD4 ＋ CD25 ＋ T cells in blood; 27 days after injection of anti-CD25 mAb, there were CD4 ＋ CD25 ＋ T cells of which percentage was lower than normal mice. 7 days after prime, depletion of CD4 ＋ CD25 ＋ T cells leaded to increased total IgE, OVA specific IgE and IgG1; 14 days after boost, depletion of CD4 ＋ CD25 ＋ T cells leaded to increased OVA specific IgE. Conclusion Depletion of CD4 ＋ CD25 ＋ T cells could effect OVA specific humoral immune response of mice immunized with OVA.
出处
《分子诊断与治疗杂志》
2011年第4期237-240,共4页
Journal of Molecular Diagnostics and Therapy
基金
国家自然科学基金(30872310)
广州市属高校科研项目(10A159)
