摘要
目的 探讨PCR扩增日本血吸虫Sj26、Sj32和Sj14-3-3抗原编码基因的诊断价值.方法 从40例慢性日本血吸虫病患者血清中提取13本血吸虫DNA,用PCR扩增Si26、Sj32和sjl4-3-3抗原编码基因.1.2%琼脂糖凝胶电泳进行鉴定,同时以21例华支睾吸虫病、13例卫氏并殖吸虫病患者血清和43例健康人血清作对照.结果 从慢性日本血吸虫病患者血清中可扩增出长度为399 bp的Sil4-3_3抗原编码基因片段,但未扩增出长度为676 bp的Si26和1270 bp的Sj32抗原编码基因片段.对照组均呈阴性反应.结论 PCR扩增Sjl4-3-3抗原编码基因可用于慢性日本血吸虫病的基因诊断.
Objective To investigate the diagnostic value of coding gene of Sj26, Sj32 and Sj14-3-3 amplified by PCR for chronic Schistosomiasis japonica. Methods The DNA was extracted from sera of 40 patients with chronic Schistosomiasis japonica, the coding gene of Sj26, Sj32 and Sj14-3-3 was amplified by PCR and identified by 1.2% agarose gel electrophoresis. DNA from the sera of 21 patients with Clonorchiasis sinensis, 13 patients with Parogonimiasis westermani and 43 healthy donors was taken as control. Results A total of 399 bp coding gene of Sj14-3-3 was amplified successfully from sera of the patients with chronic Schistosomiasis japonica,but Sj26（676 bp） and Sj32（ 1270 bp） coding gene were not obtained. Control groups were all negative. Conclusions Sj14-3-3 coding gene amplified by PCR can be used for genetic diagnosis of chronic schistosomiasis.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2011年第4期361-363,共3页
Chinese Jouranl of Endemiology
基金
重庆市科委地方病重大专项基金（2008AB5055、2008AB5008、2008AB5054）
关键词
血吸虫病
日本
聚合酶链反应
基因疗法
Schistosomiasis japonica
Polymerase chain reaction
Gene therapy
