摘要
背景:诱导多能干细胞治疗缺血性心脏病被认为是极具前景的方法,但目前仍未找到一种理想的诱导方式。目的:观察维生素C作为诱导剂对多能干细胞体外分化为心肌细胞的影响。方法:复苏小鼠诱导多能干细胞,传代培养后,采用直接悬浮培养法使小鼠诱导多能干细胞形成拟胚体,用含10-3,10-4,10-5,10-6mol/L4种不同浓度维生素C的分化培养基对其进行诱导分化,以不添加任何诱导剂作为对照组,观察各组出现搏动拟胚体的数量,计算分化比率。结果与结论:维生素C诱导小鼠诱导多能干细胞分化为心肌细胞的最佳浓度为10-4mol/L,有(57.00±3.20)%的拟胚体出现搏动,显著高于不添加任何诱导剂的对照组(5.13%±0.55)%(P<0.01)。诱导而来的心肌细胞表达心肌特异性蛋白cTnT以及α-actin。提示最佳的维生素C诱导分化条件能够促进小鼠诱导多能干细胞向心肌细胞分化,提高其分化效率。
BACKGROUND：Inducing pluripotent stem cells have been considered promising treatment for ischemic heart disease. However,an ideal inducing method has not been found yet. OBJECTIVE：To investigate the effect of vitamin C on the cardiomyocytes differentiation from mouse induced pluriptent stem cells（miPS） . METHODS：miPS were recovered,passaged and form embryoid bodies. The embryoid bodies were induced by differentiation medium containing various concentration of vitamin C（10-3,10-4,10-5,10-6 mol/L） . Control group was not treated by inducer. The number of beating embryoid bodies were calculated. RESULTS AND CONCLUSION：The best concentration of vitamin C to promote the cardiac differentiation of miPS was 10-4 mol/L. Beating area was found in 57.00%±3.20% of embryoid bodies,significantly greater than control group（5.13%±0.55%;P 0.01） . Special cardiac proteins α-actin and cTnT were detected. Results showed that vitamin C could redound differentiation of miPS into cardiomyocytes.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第23期4229-4232,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
重庆市卫生局医学科学技术研究重点项目(20090151)
课题名称"细胞或组织心脏移植的电生理研究"~~
